A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint
Por:
Gomez-Fuentes, Sandra, Hernandez-de la Fuente, Sarah, Morales-Ruiz, Valeria, Lopez-Recinos, Dina, Guevara-Salinas, Adrian, Cristina Parada-Colin, Maria, Espitia, Clara, Ochoa-Leyva, Adrian, Sanchez, Filiberto, Villalobos, Nelly, Arce-Sillas, Asiel, Hernandez, Marisela, Ivonne Mora, Silvia, Fragoso, Gladis, Sciutto, Edda, Adalid-Peralta, Laura
Publicada:
1 feb 2021
Resumen:
The flatworm Taenia solium causes human and pig cysticercosis. When
cysticerci are established in the human central nervous system, they
cause neurocysticercosis, a potentially fatal disease.
Neurocysticercosis is a persisting public health problem in rural
regions of Mexico and other developing countries of Latin America, Asia,
and Africa, where the infection is endemic. The great variability
observed in the phenotypic and genotypic traits of cysticerci result in
a great heterogeneity in the patterns of molecules secreted by them
within their host.
This work is aimed to identify and characterize cysticercal secretion
proteins of T. solium cysticerci obtained from 5 naturally infected pigs
from Guerrero, Mexico, using 2D-PAGE proteomic analysis. The isoelectric
point (IP) and molecular weight (MW) of the spots were identified using
the software ImageMaster 2D Platinum v.7.0. Since most secreted proteins
are impossible to identify by mass spectrometry (MS) due to their low
concentration in the sample, a novel strategy to predict their sequence
was applied. In total, 108 conserved and 186 differential proteins were
identified in five cultured cysts. Interestingly, we predicted the
sequence of 14 proteins that were common in four out of five cysticercus
cultures, which could be used to design vaccines or diagnostic methods
for neurocysticercosis. A functional characterization of all sequences
was performed using the algorithms SecretomeP, SignalP, and BlastKOALA.
We found a possible link between signal transduction pathways in
parasite cells and human cancer due to deregulation in signal
transduction pathways. Bioinformatics analysis also demonstrated that
the parasite release proteins by an exosome-like mechanism, which could
be of biological interest.
Author summary
Human neurocysticercosis is a major neglected tropical disease caused by
Taenia solium. As part of their natural metabolism, cysticerci release
molecules into their microenvironment within the host. Secreted
molecules could be useful in the design of vaccine candidates or
diagnostic methods, or as therapeutic targets. Unfortunately, most
secreted proteins cannot be identified by mass spectrometry sequencing
due to their low concentration in the sample. In this study, we
introduce a novel strategy to predict the sequence of T. solium secreted
proteins using 2D-PAGE proteomic analysis and the T. solium genome. This
approach allowed us to identify proteins with potential applications in
the diagnosis and/or prevention of neurocysticercosis.
Filiaciones:
Gomez-Fuentes, Sandra:
Inst Nacl Neurol & Neurocirug, Unidad Perifer Neuroinflamac, Inst Invest Biomed, Mexico City, DF, Mexico
Hernandez-de la Fuente, Sarah:
Inst Nacl Neurol & Neurocirug, Unidad Perifer Neuroinflamac, Inst Invest Biomed, Mexico City, DF, Mexico
Morales-Ruiz, Valeria:
Inst Nacl Neurol & Neurocirug, Unidad Perifer Neuroinflamac, Inst Invest Biomed, Mexico City, DF, Mexico
Lopez-Recinos, Dina:
Inst Nacl Neurol & Neurocirug, Unidad Perifer Neuroinflamac, Inst Invest Biomed, Mexico City, DF, Mexico
Guevara-Salinas, Adrian:
Inst Nacl Neurol & Neurocirug, Unidad Perifer Neuroinflamac, Inst Invest Biomed, Mexico City, DF, Mexico
Cristina Parada-Colin, Maria:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Espitia, Clara:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Ochoa-Leyva, Adrian:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Sanchez, Filiberto:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Villalobos, Nelly:
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Mexico City, DF, Mexico
Arce-Sillas, Asiel:
Inst Nacl Neurol & Neurocirug, Unidad Perifer Neuroinflamac, Inst Invest Biomed, Mexico City, DF, Mexico
Hernandez, Marisela:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Ivonne Mora, Silvia:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Fragoso, Gladis:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Sciutto, Edda:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Adalid-Peralta, Laura:
Inst Nacl Neurol & Neurocirug, Unidad Perifer Neuroinflamac, Inst Invest Biomed, Mexico City, DF, Mexico
Inst Nacl Neurol & Neurocirug, Mexico City, DF, Mexico
|