Phytophthora Root Rot Modifies the Composition of the Avocado Rhizosphere Microbiome and Increases the Abundance of Opportunistic Fungal Pathogens
Por:
Solis-Garcia, Itzel A., Ceballos-Luna, Oscar, Cortazar-Murillo, Elvis Marian, Desgarennes, Damaris, Garay-Serrano, Edith, Patino-Conde, Violeta, Guevara-Avendano, Edgar, Mendez-Bravo, Alfonso, Reverchon, Frederique
Publicada:
12 ene 2021
Resumen:
The structure and function of rhizosphere microbial communities are
affected by the plant health status. In this study, we investigated the
effect of root rot on the avocado rhizosphere microbiome, using 16S rDNA
and ITS sequencing. Furthermore, we isolated potential fungal pathogens
associated with root rot symptoms and assessed their pathogenic activity
on avocado. We found that root rot did not affect species richness,
diversity or community structure, but induced changes in the relative
abundance of several microbial taxa. Root rot increased the proportion
of Pseudomonadales and Burkholderiales in the rhizosphere but reduced
that of Actinobacteria, Bacillus spp. and Rhizobiales. An increase in
putative opportunistic fungal pathogens was also detected in the roots
of symptomatic trees; the potential pathogenicity of Mortierella sp.,
Fusarium spp., Lasiodiplodia sp. and Scytalidium sp., is reported for
the first time for the State of Veracruz, Mexico. Root rot also
potentially modified the predicted functions carried out by
rhizobacteria, reducing the proportion of categories linked with the
lipid and amino-acid metabolisms whilst promoting those associated with
quorum sensing, virulence, and antibiotic resistance. Altogether, our
results could help identifying microbial taxa associated to the disease
causal agents and direct the selection of plant growth-promoting
bacteria for the development of biocontrol microbial consortia.
Filiaciones:
Solis-Garcia, Itzel A.:
Inst Ecol AC, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super Unidad Morelia, Lab Nacl Anal & Sintesis Ecol, Morelia, Michoacan, Mexico
Ceballos-Luna, Oscar:
Inst Ecol AC, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico
Cortazar-Murillo, Elvis Marian:
Inst Ecol AC, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico
Desgarennes, Damaris:
Inst Ecol AC, Red Biodiversidad & Sistemat, Xalapa, Veracruz, Mexico
Garay-Serrano, Edith:
Inst Ecol AC, CONACYT Red Diversidad Biol Occidente Mexicano, Patzcuaro, Mexico
Patino-Conde, Violeta:
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super Unidad Morelia, Lab Nacl Anal & Sintesis Ecol, Morelia, Michoacan, Mexico
Guevara-Avendano, Edgar:
Inst Ecol AC, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico
Univ Tecnol Mixteca, Inst Agroind, Heroica Ciudad Huajuapan, Mexico
Mendez-Bravo, Alfonso:
Univ Nacl Autonoma Mexico, CONACYT Escuela Nacl Estudios Super Unidad Moreli, Lab Nacl Anal & Sintesis Ecol, Morelia, Michoacan, Mexico
Reverchon, Frederique:
Inst Ecol AC, Red Estudios Mol Avanzados, Patzcuaro, Mexico
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