Phylogenetic Identification, Diversity, and Richness of Aspergillus from Homes in Havana, Cuba
Por:
Sanchez Espinosa, Kenia C., Almaguer Chavez, Michel, Duarte-Escalante, Esperanza, Rojas Flores, Teresa Irene, Frias-De-Leon, Maria Guadalupe, Reyes-Montes, Maria del Rocio
Publicada:
1 ene 2021
Resumen:
Aspergillus is one of the most common fungal genera found indoors; it is
important because it can cause a wide range of diseases in humans.
Aspergillus species identification is based on a combination of
morphological, physiological, and molecular methods. However, molecular
methodologies have rarely been used for the identification of
environmental isolates of Aspergillus in Cuba. Therefore, the objective
of this work was to identify the species of the genus Aspergillus
obtained from houses in Havana, Cuba, through the construction of
phylogeny from a partial sequence of the benA gene region, and to
analyze the diversity and richness of Aspergillus in the studied
municipalities. Isolates of Aspergillus spp. included in this study
presented the typical macro- and micromorphology described for the
genus. According to this polyphasic characterization, A. niger, A.
flavus, A. welwitschiae, A. heteromorphus, A. sydowii, A. tamarii, A.
fumigatus, A. clavatus, and A. tubingensis were the most abundant
species. Most of the identified species constitute new records for
outdoor and indoor environments in Cuba and contribute to the knowledge
of fungal biodiversity in the country. These results constitute an alert
for the health authorities of the country, since prolonged exposure of
the inhabitants to Aspergillus spores can cause severe persistent
asthma, among other diseases.
Filiaciones:
Univ La Habana, Fac Biol, Dept Microbiol & Virol, 25,455 Entre I&J, Havana 10400, Cuba
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
Hosp Reg Alta Especialidad Ixtapaluca, Carretera Fed Mexico Puebla Km 34-5, Pueblo De Zoquiapan 56530, Ixtapaluca, Mexico
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