Differential miRNA Expression in Human Macrophage-Like Cells Infected with Histoplasma capsulatum Yeasts Cultured in Planktonic and Biofilm Forms
Por:
Pitangui, Nayla de Souza, de Lacorte Singulani, Junya, Sardi, Janaina de Cassia Orlandi, de Souza, Paula Carolina, Rodriguez-Arellanes, Gabriela, Garcia-Perez, Blanca Estela, Enguita, Francisco Javier, Pavan, Fernando R., TAYLOR, MARIA LUCIA, Mendes-Giannini, Maria Jose Soares, Fusco-Almeida, Ana Marisa
Publicada:
1 ene 2021
Resumen:
Histoplasma capsulatum affects healthy and immunocompromised
individuals, sometimes causing a severe disease. This fungus has two
morphotypes, the mycelial (infective) and the yeast (parasitic) phases.
MicroRNAs (miRNAs) are small RNAs involved in the regulation of several
cellular processes, and their differential expression has been
associated with many disease states. To investigate miRNA expression in
host cells during H. capsulatum infection, we studied the changes in the
miRNA profiles of differentiated human macrophages infected with yeasts
from two fungal strains with different virulence, EH-315 (high
virulence) and 60I (low virulence) grown in planktonic cultures, and
EH-315 grown in biofilm form. MiRNA profiles were evaluated by means of
reverse transcription-quantitative polymerase chain reaction using a
commercial human miRNome panel. The target genes of the differentially
expressed miRNAs and their corresponding signaling pathways were
predicted using bioinformatics analyses. Here, we confirmed biofilm
structures were present in the EH-315 culture whose conditions
facilitated producing insoluble exopolysaccharide and intracellular
polysaccharides. In infected macrophages, bioinformatics analyses
revealed especially increased (hsa-miR-99b-3p) or decreased
(hsa-miR-342-3p) miRNAs expression levels in response to infection with
biofilms or both growth forms of H. capsulatum yeasts, respectively. The
results of miRNAs suggested that infection by H. capsulatum can affect
important biological pathways of the host cell, targeting two genes: one
encoding a protein that is important in the cortical cytoskeleton; the
other, a protein involved in the formation of stress granules. Expressed
miRNAs in the host's response could be proposed as new therapeutic
and/or diagnostic tools for histoplasmosis.
Filiaciones:
Pitangui, Nayla de Souza:
Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP, Brazil
Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Cellular & Mol Biol, BR-14049900 Ribeirao Preto, SP, Brazil
de Lacorte Singulani, Junya:
Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP, Brazil
Sardi, Janaina de Cassia Orlandi:
Univ Fed Mato Grosso do Sul, Sch Pharmaceut Sci Food & Nutr, BR-79070900 Campo Grande, MS, Brazil
de Souza, Paula Carolina:
Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP, Brazil
Rodriguez-Arellanes, Gabriela:
UNAM Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Unidad Micol, Mexico City 04510, DF, Mexico
Garcia-Perez, Blanca Estela:
IPN Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City 11340, DF, Mexico
Enguita, Francisco Javier:
Univ Lisbon, Fac Med, Inst Med Mol, P-1649028 Lisbon, Portugal
Pavan, Fernando R.:
Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP, Brazil
TAYLOR, MARIA LUCIA:
UNAM Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Unidad Micol, Mexico City 04510, DF, Mexico
Mendes-Giannini, Maria Jose Soares:
Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP, Brazil
Fusco-Almeida, Ana Marisa:
Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP, Brazil
|