Serotyping versus genotyping in infected sheep and goats with small ruminant lentiviruses
Por:
Acevedo Jimenez, Gabriel Eduardo, Tortora Perez, Jorge Luis, Rodriguez Murillo, Cecilia, Arellano Reynoso, Beatriz, Ramirez Alvarez, Hugo
Publicada:
1 ene 2021
Resumen:
Despite SRLV infection being endemic in Mexico, there is little
information regarding which genotypes are present. We compared
serotyping and PCR-sequencing results from sheep and goats infected with
SRLV. We separated plasma and peripheral blood leukocytes (PBL) from
1940 blood samples from sheep and goats from 12 states across Mexico. To
detect SRLV infection, we tested plasma samples using two commercial
ELISA kits (VMRD and Eradikit SRLV Screening). Then, we semtyped the
infecting virus (A/ B) using Eradikit SRLV Genotyping. PBL DNA was used
to detect the proviral genome via PCR. Positive amplicons were sequenced
to identify viral genotypes using a phylogenetic analysis. Also, we
analysed for residues differences in the sequences of a capsid epitope
between genotypes. The serological results indicated a higher detection
of seropositive animals using the VMRD ELISA compared to Eradikit, with
21 % and 15.3 % more in sheep and goats respectively. Only 25.7 % of
the ELISA serotyping results matched those from PCR-sequencing.
PCR-sequencing was able to identify genotype A, B and coinfections in
animals classified as indeterminate by the ELISA test. This lack of
sensitivity may be related to the lack of epitopes from the matrix and
transmembrane peptides used by ELISA screening. Sequences analysis
revealed that SRLVs found in sheep cluster with genetic subtypes A2 and
B1, while those in goats cluster with subtypes A1 and B1. Serotyping did
not prove to be an adequate method for predicting the viral genotype (A
and / or B) in infections caused by SRLV.
Filiaciones:
Acevedo Jimenez, Gabriel Eduardo:
Univ Nacl Autonoma Mexico, Fac Higher Educ, Virol Genet & Mol Biol Lab, Cuautitlan,Vet Med, Campus 4,Km 2-5 Ctra Cuautitlan Teoloyucan, San Sebastian Xhala 54714, Cuautitlan Izca, Mexico
Tortora Perez, Jorge Luis:
Univ Nacl Autonoma Mexico, Fac Higher Educ, Virol Genet & Mol Biol Lab, Cuautitlan,Vet Med, Campus 4,Km 2-5 Ctra Cuautitlan Teoloyucan, San Sebastian Xhala 54714, Cuautitlan Izca, Mexico
Rodriguez Murillo, Cecilia:
Univ Nacl Autonoma Mexico, Fac Higher Educ, Virol Genet & Mol Biol Lab, Cuautitlan,Vet Med, Campus 4,Km 2-5 Ctra Cuautitlan Teoloyucan, San Sebastian Xhala 54714, Cuautitlan Izca, Mexico
Arellano Reynoso, Beatriz:
Univ Nacl Auton Mexico, Fac Med Vet & Zootecnia, Dept Microbiol & Inmunol, Ave Univ 3000, Mexico City 04510, DF, Mexico
Ramirez Alvarez, Hugo:
Univ Nacl Autonoma Mexico, Fac Higher Educ, Virol Genet & Mol Biol Lab, Cuautitlan,Vet Med, Campus 4,Km 2-5 Ctra Cuautitlan Teoloyucan, San Sebastian Xhala 54714, Cuautitlan Izca, Mexico
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