Unravelling Chemical Composition of Agave Spines: News from Agave fourcroydes Lem.
Por:
Moran-Velizquez, Dalia C., Monribot-Villanueva, Juan L., Bourdon, Matthieu, Tang, John Z., LOpez-Rosas, Itzel, Maceda-LOpez, Luis E., Villalpando-Aguilar, Jose L., Rodriguez-Lopez, Lorena, Gauthier, Adrien, Trejo, Laura, Azadi, Parastoo, Vilaplana, Francisco, Guerrero-Analco, Jose A., Alatorre-Cobos, Fulgencio
Publicada:
1 dic 2020
Resumen:
Spines are key plant modifications developed to deal against herbivores;
however, its physical structure and chemical composition have been
little explored in plant species. Here, we took advantage of
high-throughput chromatography to characterize chemical composition of
Agave fourcroydes Lem. spines, a species traditionally used for fiber
extraction. Analyses of structural carbohydrate showed that spines have
lower cellulose content than leaf fibers (52 and 72%, respectively) but
contain more than 2-fold the hemicellulose and 1.5-fold pectin. Xylose
and galacturonic acid were enriched in spines compared to fibers. The
total lignin content in spines was 1.5-fold higher than those found in
fibers, with elevated levels of syringyl (S) and guaiacyl (G) subunits
but similar S/G ratios within tissues. Metabolomic profiling based on
accurate mass spectrometry revealed the presence of phenolic compounds
including quercetin, kaempferol, (+)-catechin, and (-)-epicatechin in A.
fourcroydes spines, which were also detected in situ in spines tissues
and could be implicated in the color of these plants' structures.
Abundance of (+)-catechins could also explain proanthocyanidins found in
spines. Agave spines may become a plant model to obtain more insights
about cellulose and lignin interactions and condensed tannin deposition,
which is valuable knowledge for the bioenergy industry and development
of naturally dyed fibers, respectively.
Filiaciones:
Moran-Velizquez, Dalia C.:
Colegio Postgrad, Campus Campeche, Sihochac 24450, Campeche, Mexico
Monribot-Villanueva, Juan L.:
Inst Ecol AC, Red Estudios Mol Avanzados REMAV, Carretera Antigua Coatepec 351, Xalapa 91070, Veracruz, Mexico
Bourdon, Matthieu:
Univ Cambridge, Sainsbury Lab, Cambridge CB2 1LR, England
Tang, John Z.:
Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
LOpez-Rosas, Itzel:
Colegio Postgrad, Campus Campeche, Sihochac 24450, Campeche, Mexico
Maceda-LOpez, Luis E.:
Colegio Postgrad, Campus Campeche, Sihochac 24450, Campeche, Mexico
Villalpando-Aguilar, Jose L.:
Inst Tecnol China, Tecnol Nacl Mexico, Calle 11 Entre 22 & 28, China 24050, Mexico
Rodriguez-Lopez, Lorena:
Colegio Postgrad, Campus Campeche, Sihochac 24450, Campeche, Mexico
Gauthier, Adrien:
UniLaSalle AGHYLE Res Unit 2018 C101, 3 Rue Tronquet CS 40118-76134, F-76134 Mont St Aignan, France
Trejo, Laura:
UNAM, Lab Biodiversidad & Cult Tejidos Vegetales, Inst Biol, Santa Cruz 90640, Tlaxcala, Mexico
Azadi, Parastoo:
Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
Vilaplana, Francisco:
KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Chem, Div Glycosci,Sch Engn Sci Chem, SE-10044 Stockholm, Sweden
Guerrero-Analco, Jose A.:
Inst Ecol AC, Red Estudios Mol Avanzados REMAV, Carretera Antigua Coatepec 351, Xalapa 91070, Veracruz, Mexico
Alatorre-Cobos, Fulgencio:
Colegio Postgrad, Campus Campeche, Sihochac 24450, Campeche, Mexico
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