Antihypertensive and vasorelaxant mode of action of the ethanol-soluble extract from Tagetes lucida Cav. aerial parts and its main bioactive metabolites
Por:
Estrada-Soto, Samuel, Gonzalez-Trujano, Ma. Eva, Rendon-Vallejo, Priscila, Arias-Duran, Luis, Avila-Villarreal, Gabriela, Villalobos-Molina, Rafael
Publicada:
10 feb 2021
Resumen:
Ethnopharmacological relevance: Tagetes lucida Cav. commonly known as
``yauhtli'' or ``peric ` on'' is used in Mexican traditional
medicine for the treatment of anxiety, depressant diseases, pain,
hypertension, among others. Aim: To evaluate the antihypertensive and
vasorelaxant modes of action of a crude ethanolic extract from T. lucida
aerial parts and to isolate the bioactive compounds.
Materials and methods: Ethanolic extract was tested in an in vivo assay
in SHR rats by intragastric administration at 10 and 100 mg/kg dosages,
to measure and to compare hemodynamic parameters like diastolic and
systolic blood pressure and heart rate. Also, extract (3.03-1000 mu
g/ml), fractions (3.03-1000 mu g/ml) and pure isolated compounds
(1.75-550 mu M) were evaluated on isolated aortic rings contracted with
noradrenaline (0.1 mu M) to determine their vasorelaxant effect and
extract-mode of action.
Results: Ethanolic extract of T. lucida lowered systolic and diastolic
blood pressure on SHR rats without heart rate modification (P 0.05).
Moreover, the extract showed concentration-dependent relaxant effect in
a partially endothelium-dependent manner (P < 0.05), through NO/cGMP
system activation and calcium channel blockade. 6,7,8-trimethoxycoumarin
(1), 6,7-dimethoxycoumarin (2), and 7-methoxycoumarin (3) from T. lucida
are the main bioactive compounds of the extract and showed significant
vasorelaxant activity.
Conclusions: Results provide evidence and endorsed the antihypertensive
properties attributed to T. lucida in traditional medicine, which is
produced by vasorelaxant effect mainly through multitarget NO/cGMP
system activation and calcium channel blockade. Coumarin derivatives 1,
2 and 3 are the responsible of the vasorelaxant activity.
Filiaciones:
Estrada-Soto, Samuel:
Univ Autonoma Estado Morelos, Fac Farm, Cuernavaca, Morelos, Mexico
Gonzalez-Trujano, Ma. Eva:
Inst Nacl Psiquiatria Ramon Fuente Muniz, Direcc Invest Neurociencias, Lab Neurofarmacol Prod Nat, Mexico City, DF, Mexico
Rendon-Vallejo, Priscila:
Univ Autonoma Estado Morelos, Fac Farm, Cuernavaca, Morelos, Mexico
Arias-Duran, Luis:
Univ Autonoma Estado Morelos, Fac Farm, Cuernavaca, Morelos, Mexico
Avila-Villarreal, Gabriela:
Univ Autonoma Nayarit, Ctr Nayarita Innovac & Transferencia Tecnol, Unidad Especializada I D I Calidad Alimentos & Pr, Nayarit, Mexico
Villalobos-Molina, Rafael:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Toluca, Estado De Mexic, Mexico
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