Dental Pulp Mesenchymal Stem Cells as a Treatment for Periodontal Disease in Older Adults
Por:
Hernandez-Monjaraz, Beatriz, Santiago-Osorio, Edelmiro, Ledesma-Martinez, Edgar, Aguiniga-Sanchez, Itzen, Sosa-Hernandez, Norma Angelica, Mendoza-Nunez, Victor Manuel
Publicada:
18 ago 2020
Resumen:
Periodontal disease (PD) is one of the main causes of tooth loss and is
related to oxidative stress and chronic inflammation. Although different
treatments have been proposed in the past, the vast majority do not
regenerate lost tissues. In this sense, the use of dental pulp
mesenchymal stem cells (DPMSCs) seems to be an alternative for the
regeneration of periodontal bone tissue. A quasi-experimental study was
conducted in a sample of 22 adults between 55 and 64 years of age with
PD, without uncontrolled systemic chronic diseases. Two groups were
formed randomly: (i) experimental group (EG)n=11, with a treatment based
on DPMSCs; and a (ii) control group (CG)n=11, without a treatment of
DPMSCs. Every participant underwent clinical and radiological
evaluations and measurement of bone mineral density (BMD) by tomography.
Saliva samples were taken as well, to determine the total concentration
of antioxidants, superoxide dismutase (SOD), lipoperoxides, and
interleukins (IL), before and 6 months after treatment. All subjects
underwent curettage and periodontal surgery, the EG had a collagen
scaffold treated with DPMSCs, while the CG only had the collagen
scaffold placed. The EG with DPMSCs showed an increase in the BMD of the
alveolar bone with a borderline statistical significance (baseline
638.82 +/- 181.7 vs. posttreatment781.26 +/- 162.2HU, p=0.09). Regarding
oxidative stress and inflammation markers, salivary SOD levels were
significantly higher in EG (baseline1.49 +/- 0.96vs.2.14 +/- 1.12
U/Lposttreatment,p<0.05) meanwhile IL1 beta levels had a decrease
(baseline 1001.91 +/- 675.5 vs. posttreatment 722.3 +/- 349.4 pg/ml,
p<0.05). Our findings suggest that a DPMSCs treatment based on DPMSCs
has both an effect on bone regeneration linked to an increased SOD and
decreased levels of IL1 beta in aging subjects with PD.
Filiaciones:
Hernandez-Monjaraz, Beatriz:
Univ Nacl Autonoma Mexico, FES Zaragoza, Res Unit Gerontol, Mexico City 09230, DF, Mexico
Santiago-Osorio, Edelmiro:
Univ Nacl Autonoma Mexico, FES Zaragoza, Res Unit Cell Differentiat & Canc, Haematopoiesis & Leukaemia Lab, Mexico City 09230, DF, Mexico
Ledesma-Martinez, Edgar:
Univ Nacl Autonoma Mexico, FES Zaragoza, Res Unit Cell Differentiat & Canc, Haematopoiesis & Leukaemia Lab, Mexico City 09230, DF, Mexico
Aguiniga-Sanchez, Itzen:
Univ Nacl Autonoma Mexico, FES Zaragoza, Res Unit Cell Differentiat & Canc, Haematopoiesis & Leukaemia Lab, Mexico City 09230, DF, Mexico
Sosa-Hernandez, Norma Angelica:
Univ Nacl Autonoma Mexico, FES Zaragoza, Res Unit Cell Differentiat & Canc, Haematopoiesis & Leukaemia Lab, Mexico City 09230, DF, Mexico
Mendoza-Nunez, Victor Manuel:
Univ Nacl Autonoma Mexico, FES Zaragoza, Res Unit Gerontol, Mexico City 09230, DF, Mexico
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