First evidence of bovine immunodeficiency virus infection in Mexican cattle
Por:
González-Fernández V.D., Tortora Perez, Jorge Luis, Garcia Flores, Maria Martha, Aguilar Setien, Jose Alvaro, Ramirez Alvarez, Hugo
Publicada:
1 sep 2020
Ahead of Print:
1 ene 2020
Resumen:
This study set out to identify the presence of bovine immunodeficiency virus (BIV) in animals geographically located in Mexico. BIV was first discovered in the United States in a dairy cow with persistent lymphocytosis, lymphoid hyperplasia and lymphocytic encephalitis. Many studies indicate that BIV infection is globally distributed, but its presence in Mexico remains unknown. We collected 1,168 heparinized blood samples from cattle in ten states across the Mexican Republic, then separated plasma using centrifugation and tested for antibodies against BIV. We used an indirect ELISA based on the use of a synthetic peptide derived from transmembrane glycoprotein (gp45/TM). In order to identify the viral genome, we designed a synthetic gene as a PCR control, as well as a pair of oligonucleotides for amplifying a 519 bp product of the env gene which encodes the surface protein. Positive amplicons were purified and subjected to nucleotide sequencing. A total of 189 (28.94%) tested plasma samples suggest the presence of specific anti-BIV antibodies in all states studied except for Chiapas. Additionally, PCR results identified six positive cows in the states of Puebla and Coahuila. BIV in these cows was confirmed via nucleotide sequencing and in silico analysis of these samples. This is the first report of the presence of BIV in Mexican cattle. © 2020 Blackwell Verlag GmbH
Filiaciones:
González-Fernández V.D.:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, National Autonomous University of Mexico, Cuautitlán Izcalli, Mexico
Tortora Perez, Jorge Luis:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, National Autonomous University of Mexico, Cuautitlán Izcalli, Mexico
Univ Nacl Autonoma Mexico, Fac Higher Educ, Virol Genet & Mol Biol Lab, Vet Med, Cuautitlan, Mexico
Garcia Flores, Maria Martha:
Laboratory of Immunovirology, Medical Research in Immunology Unit, Pediatric Hospital, National Medical Center XXI Century, Mexican Institute of Social Security, Mexico City, Mexico
Mexican Inst Social Secur, Natl Med Ctr 21 Century, Pediat Hosp, Lab Immunovirol,Med Res Immunol Unit, Mexico City, DF, Mexico
Aguilar Setien, Jose Alvaro:
Laboratory of Immunovirology, Medical Research in Immunology Unit, Pediatric Hospital, National Medical Center XXI Century, Mexican Institute of Social Security, Mexico City, Mexico
Mexican Inst Social Secur, Natl Med Ctr 21 Century, Pediat Hosp, Lab Immunovirol,Med Res Immunol Unit, Mexico City, DF, Mexico
Ramirez Alvarez, Hugo:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, National Autonomous University of Mexico, Cuautitlán Izcalli, Mexico
Univ Nacl Autonoma Mexico, Fac Higher Educ, Virol Genet & Mol Biol Lab, Vet Med, Cuautitlan, Mexico
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