Phenylpropanoids Are Connected to Cell Wall Fortification and Stress Tolerance in Avocado Somatic Embryogenesis
Por:
Olivares-García C.A., Mata-Rosas M., Peña-Montes C., Quiroz-Figueroa F., Segura-Cabrera A., Shannon L.M., Loyola-Vargas V.M., Monribot-Villanueva J.L., Elizalde-Contreras J.M., Ibarra-Laclette E., Ramirez-Vázquez M., Guerrero-Analco J.A., Ruiz-May E.
Publicada:
1 ene 2020
Resumen:
Somatic embryogenesis (SE) is a valuable model for understanding the mechanism of plant embryogenesis and a tool for the mass production of plants. However, establishing SE in avocado has been complicated due to the very low efficiency of embryo induction and plant regeneration. To understand the molecular foundation of the SE induction and development in avocado, we compared embryogenic (EC) and non-embryogenic (NEC) cultures of two avocado varieties using proteomic and metabolomic approaches. Although Criollo and Hass EC exhibited similarities in the proteome and metabolome profile, in general, we observed a more active phenylpropanoid pathway in EC than NEC. This pathway is associated with the tolerance of stress responses, probably through the reinforcement of the cell wall and flavonoid production. We could corroborate that particular polyphenolics compounds, including p-coumaric acid and t-ferulic acid, stimulated the production of somatic embryos in avocado. Exogen phenolic compounds were associated with the modification of the content of endogenous polyphenolic and the induction of the production of the putative auxin-a, adenosine, cellulose and 1,26-hexacosanediol-diferulate. We suggest that in EC of avocado, there is an enhanced phenylpropanoid metabolism for the production of the building blocks of lignin and flavonoid compounds having a role in cell wall reinforcement for tolerating stress response. Data are available at ProteomeXchange with the identifier PXD019705.
Filiaciones:
Olivares-García C.A.:
Red de Manejo Biotecnológico de Recursos, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351 ,Veracruz CP 91073, Mexico
Tecnológico Nacional de México, Instituto Tecnológico de Veracruz, Unidad de Investigación y Desarrollo en Alimentos, Veracruz CP 91897, Mexico
Mata-Rosas M.:
Red de Manejo Biotecnológico de Recursos, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351 ,Veracruz CP 91073, Mexico
Peña-Montes C.:
Tecnológico Nacional de México, Instituto Tecnológico de Veracruz, Unidad de Investigación y Desarrollo en Alimentos, Veracruz CP 91897, Mexico
Quiroz-Figueroa F.:
Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional-Unidad Sinaloa, Boulevard Juan de Dios Bátiz Paredes # 250, Col. San Joachin, Guasave, Sinaloa 81101, Mexico
Segura-Cabrera A.:
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus ,Hinxton, Cambridgeshire, CB10 1SD, United Kingdom
Shannon L.M.:
Department of Horticultural Science, University of MinnesotaSaint Paul, United States
Loyola-Vargas V.M.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Yucatán CP 97205Mérida, Mexico
Monribot-Villanueva J.L.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351 ,Veracruz CP 91073, Mexico
Elizalde-Contreras J.M.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351 ,Veracruz CP 91073, Mexico
Ibarra-Laclette E.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351 ,Veracruz CP 91073, Mexico
Ramirez-Vázquez M.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351 ,Veracruz CP 91073, Mexico
Guerrero-Analco J.A.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351 ,Veracruz CP 91073, Mexico
Ruiz-May E.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351 ,Veracruz CP 91073, Mexico
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