Human serum proteins bind to Sporothrix schenckii conidia with differential effects on phagocytosis
Por:
Guzman Beltran, Silvia, Sanchez Morales, Jazmin, Gonzalez Canto, Augusto, Escalona Montaño A., Torres Guerrero, Haydee
Publicada:
1 mar 2021
Ahead of Print:
1 ene 2020
Categoría:
Microbiology
Resumen:
Serum is an important source of proteins that interact with pathogens.
Once bound to the cell surface, serum proteins can stimulate the innate
immune system. The phagocytosis of Sporothrix schenckii conidia by human
macrophages is activated through human serum opsonisation. In this
study, we have attempted to characterise human blood serum proteins that
bind to the cell wall of S. schenckii conidia. We systematically
observed the same four proteins independent of the plasma donor:
albumin, serum amyloid protein (SAP), alpha-1 antitrypsin (AAT), and
transferrin were identified with the help of tandem mass spectrometry.
Phagocytosis depended on the concentration of the SAP or alpha-1
antitrypsin that was used to opsonise the conidia; however, transferrin
or albumin did not have any effect on conidia internalisation. The
presence of mannose did not affect macrophage phagocytosis of the
conidia opsonised with SAP or alpha-1 antitrypsin, which suggests that
these proteins are not recognised by the mannose receptor.
Filiaciones:
Departamento de Investigación en Microbiología, Instituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas”, Ciudad de México, 14502, Mexico
Unidad de Investigación en Medicina Experimental, Micología Básica, Facultad de Medicina, Universidad Nacional Autónoma de México, Hospital General de México “Dr. Eduardo Liceaga”, Ciudad de México, Mexico
Unidad de Investigación en Medicina Experimental, Patología Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Hospital General de México “Dr. Eduardo Liceaga”, Ciudad de México, Mexico
Unidad Periferica de la Facultad de Medicina, Unidad de Investigación en Medicina Traslacional. Inmunobioquímica Molecular y Cardiopatías, Ciudad de México, 14080, Mexico
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