The 50 kDa metalloproteinase TvMP50 is a zinc-mediated Trichomonas vaginalis virulence factor
Por:
Puente-Rivera J., Villalpando J.L., Villalobos-Osnaya A., Vázquez-Carrillo L.I., León-Ávila G., Ponce-Regalado M.D., López-Camarillo C., Elizalde-Contreras J.M., Ruiz-May E., Arroyo R., Alvarez-Sánchez M.E.
Publicada:
1 ene 2017
Resumen:
Trichomonas vaginalis is a protozoan parasite that can adapt to the trichomonicidal Zn2+ concentrations of the male urogenital tract microenvironment. This adaptation is mediated by molecular mechanisms, including proteinase expression, that are regulated by cations such as Zn2+. Herein, we characterized the previously identified 50 kDa metalloproteinase aminopeptidase P (M24 family) member TvMP50 as a new Zn2+-mediated parasite virulence factor. Quantitative RT-PCR and indirect immunofluorescence assays corroborated the positive regulation of both mp50 gene expression and native TvMP50 protein overexpression in the cytoplasm and secretion products of parasites grown in the presence of Zn2+. Furthermore, this active metalloproteinase was characterized as a new virulence factor by assaying cytotoxicity toward prostatic DU145 cell monolayers as well as the inhibition of parasite and secreted soluble protein proteolytic activity in the 50 kDa proteolytic region by the specific metalloproteinase inhibitor 1,10-phenanthroline and the chelating agents EDTA and EGTA. Parasite and secreted soluble protein cytotoxicity toward DU145 cells were reduced by treatment with an a-rTvMP50 polyclonal antibody. Our results show that the metalloproteinase TvMP50 is a new virulence factor modulated by Zn2+, which is present during male trichomoniasis, possibly explaining T. vaginalis survival even within the adverse conditions of the male urogenital microenvironment. © 2017 Elsevier B.V.
Filiaciones:
Puente-Rivera J.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico (UACM), Mexico
Villalpando J.L.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico (UACM), Mexico
Villalobos-Osnaya A.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico (UACM), Mexico
Vázquez-Carrillo L.I.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico (UACM), Mexico
León-Ávila G.:
Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Miguel Hidalgo, Ciudad de México, 11340, Mexico
Ponce-Regalado M.D.:
Departamento de Clínicas, Centro Universitario de los Altos, Universidad de Guadalajara, Tepatitlán de MorelosJalisco, Mexico
López-Camarillo C.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico (UACM), Mexico
Elizalde-Contreras J.M.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Clúster BioMimic®, Carretera Antigua a Coatepec 351, Congregación el Haya, Xalapa, Veracruz 91070, Mexico
Ruiz-May E.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Clúster BioMimic®, Carretera Antigua a Coatepec 351, Congregación el Haya, Xalapa, Veracruz 91070, Mexico
Arroyo R.:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN # 2508, Col. San Pedro Zacatenco, Deleg. Gustavo A. Madero, Ciudad de México, CP 07360, Mexico
Alvarez-Sánchez M.E.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico (UACM), Mexico
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