Dietary Biotin Supplementation Impairs Testis Morphology and Sperm Quality
Por:
Pasten-Hidalgo, Karina, Riveron-Negrete, Leticia, Sicilia-Argumedo, Gloria, Canul-Medina, Gustavo, Salazar-Anzures, Tonatiuh, Tapia-Rodriguez, Miguel, Hernandez-Gonzalez, Enrique O., Roa-Espitia A.L., Cedillo-Pelaez, Carlos, Fernandez-Mejia, Cristina
Publicada:
1 may 2020
Ahead of Print:
1 oct 2019
Resumen:
Supplements containing pharmacological concentrations of biotin are
commercially available over the counter. Classical toxicity studies have
considered biotin administration as harmless; however, recent
investigations have shown that biotin supplementation modifies tissue
morphology without changes in toxicity markers, raising concerns about
the consequences of morphological changes on tissues' functions and the
safety of pharmacological concentrations of the vitamin. Testes are very
sensitive to toxicants, and testicular histology is a reliable method to
study its function. In this work, we investigated the effects of dietary
biotin supplementation on testis morphology and spermatogenesis function
using an experimental model, in which we have not observed unfavorable
effects on other tissue functions or toxicity markers. Male BALB/cAnNHsd
mice were fed a control or a biotin-supplemented diet (1.76 or 97.7 mg
biotin/kg diet) for 8 weeks. Compared to the control group, the
biotin-supplemented mice presented remarkable testis morphology changes,
including increased spermatogonia layers; the cellular mechanism
involved is related to increased proliferation. Sperm count and serum
testosterone levels were not affected, but spermatozoa motility and
morphology were significantly impaired in the biotin-supplemented mice.
These results caution against the use of supplements with high
concentrations of biotin and indicate that biotin's pharmacological
effects on morphology need to be considered in toxicological studies.
Filiaciones:
Cátedra CONACYT, Instituto Nacional de Pediatría, Mexico City, Mexico
Unidad de Genética de la Nutrición, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Instituto Nacional de Pediatría, Mexico City, Mexico
Unidad de Microscopía, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico
Departamento de Biología Celular, CINVESTAV-IPN, San-Pedro Zacatenco, Mexico City, Mexico
Departamento de Inmunología Experimental, Instituto Nacional de Pediatría, Mexico City, Mexico
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