Isolation and characterization of microsatellite loci in Astroniumgraveolens (Anacardiaceae) and cross amplification in related species
Por:
Sanchez-Gomez, Karman F., Cristobal-Perez, E. Jacob, Harvey, Nick, Quesada, Mauricio
Publicada:
1 may 2020
Resumen:
Microsatellite markers are a useful genetic tool to answer ecological
and conservation genetics questions. Microsatellite primers were
developed and characterized to evaluate forest fragmentation effects on
genetic structure, diversity and gene flow patterns in the dioecious
tropical tree Astroniumgraveolens (Anacardiaceae). Using genomic library
enrichment, sixteen microsatellite loci were developed for A.graveolens.
Polymorphism was evaluated in 80 individuals from four localities in
Mexico. The overall number of alleles detected was 63, average alleles
per locus 3.9 with a range from one to 11 per locus. Cross amplification
trails on related species in the Anacardiaceae family: Spondiaspurpurea
and Amphipterygiumadstringens, achieved successful amplification for all
primers. Microsatellite markers described here are the first to be
characterized for A.graveolens. These genetic markers will be a useful
tool to assess the genetic consequences of habitat fragmentation and
selective logging on A.graveolens populations. Cross amplification
success on S.purpureaandA.adstringens suggest that they may be used for
population genetic studies in other species within the family.
Filiaciones:
Sanchez-Gomez, Karman F.:
Univ Nacl Autonoma Mexico, Inst Invest Ecosistemas & Sustentabilidad, Morelia, Michoacan, Mexico
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super, Unidad Morelia, Lab Nacl Anal & Sintesis Ecol LANASE, Morelia, Michoacan, Mexico
Cristobal-Perez, E. Jacob:
Univ Nacl Autonoma Mexico, Inst Invest Ecosistemas & Sustentabilidad, Morelia, Michoacan, Mexico
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super, Unidad Morelia, Lab Nacl Anal & Sintesis Ecol LANASE, Morelia, Michoacan, Mexico
Harvey, Nick:
Genet Marker Serv, Brighton, E Sussex, England
Quesada, Mauricio:
Univ Nacl Autonoma Mexico, Inst Invest Ecosistemas & Sustentabilidad, Morelia, Michoacan, Mexico
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super, Unidad Morelia, Lab Nacl Anal & Sintesis Ecol LANASE, Morelia, Michoacan, Mexico
|