Effect of autologous transplant of peripheral blood mononuclear cells in combination with proangiogenic factors during experimental revascularization of lower limb ischemia
Por:
Padilla, Luis, Arguero-Sanchez, Ruben, Rodriguez-Trejo, Juan Miguel, Carranza-Castro, Pilar Hazel, Suarez-Cuenca, Juan Antonio, Polaco-Castillo, Jaime, DiSilvio-Lopez, Mauricio, Lopez-Gutierrez, Javier, Olguin-Juarez, Horacio, Hernandez-Patricio, Alejandro, Vera-Gomez, Eduardo, Gomez-Calderon, Alan De Jesus, Tellez-Gonzalez, Mario Antonio, Mondragon-Teran, Paul
Publicada:
1 abr 2020
Resumen:
Peripheral blood mononuclear cells (PBMCs) contain a cell fraction of
mononuclear progenitor cells (MPCs), which own significant angiogenic
potential. Autologous transplant of PBMC and/or platelet-rich plasma
(PRP) promotes endothelial cells differentiation in experimental lower
limb ischemia, which is considered a safe and effective strategy to
support revascularization, either in animal models or clinical trials.
In addition, thrombin has been proposed to enrich biological scaffolds,
hence increasing MPC viability after intramuscular administration,
whereas proangiogenic mediators such as vascular endothelial growth
factor (VEGF), tumor necrosis factor alpha (TNF-alpha), inhibitor of the
plasminogen activator-1 (PAI-1), and chemokine (CXCL1; GRO-alpha)
participate in the endothelial response to ischemia, through their
proangiogenic effects over endothelial cells proliferation, survival,
migration, endothelial integrity maintenance, and physiologic vascular
response to injury. In the present study, we describe the effect of
autologous PBMCs transplant and PRP, either with or without thrombin,
over proangiogenic mediators (measured by enzyme-linked immunosorbent
assay) and revascularization response (angiographic vascular pattern at
30 days after vascular occlusion) in a rat model of lower limb ischemia.
The group treated with PBMC + PRP significantly induced PAI-1, an effect
that was prevented by the addition of thrombin. Furthermore, treatment
with PBMC + PRP + thrombin resulted in the induction of VEGF. GRO-alpha
showed a sensitive induction of all proangiogenic mediators. All
treatments significantly stimulated revascularization, according to
angiographic assessment, whereas higher effect was observed with PBMC +
PRP treatment (p < .0001). In conclusion, autologous PBMC transplant
stimulates revascularization during experimental ischemia of the lower
limb, whereas particular effects over proangiogenic and fibrinolytic
mediators may be attributed to PBMCs and its combination with PRP and
thrombin.
Filiaciones:
Padilla, Luis:
Ctr Med Nacl 20 Noviembre, ISSSTE, Dept Expt Surg, Mexico City, DF, Mexico
Arguero-Sanchez, Ruben:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
Rodriguez-Trejo, Juan Miguel:
Ctr Med Nacl 20 Noviembre, ISSSTE, Dept Angiol & Vasc Surg, Mexico City, DF, Mexico
Carranza-Castro, Pilar Hazel:
Ctr Med Nacl 20 Noviembre, ISSSTE, Dept Expt Surg, Mexico City, DF, Mexico
Suarez-Cuenca, Juan Antonio:
Ctr Med Nacl 20 Noviembre, ISSSTE, Lab Expt Metab & Clin Res, Mexico City, DF, Mexico
Polaco-Castillo, Jaime:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
DiSilvio-Lopez, Mauricio:
Ctr Med Nacl 20 Noviembre, ISSSTE, Dept Expt Surg, Mexico City, DF, Mexico
Lopez-Gutierrez, Javier:
Ctr Med Nacl 20 Noviembre, ISSSTE, Dept Expt Surg, Mexico City, DF, Mexico
Olguin-Juarez, Horacio:
Ctr Med Nacl 20 Noviembre, ISSSTE, Dept Expt Surg, Mexico City, DF, Mexico
Hernandez-Patricio, Alejandro:
Ctr Med Nacl 20 Noviembre, ISSSTE, Lab Expt Metab & Clin Res, Mexico City, DF, Mexico
Vera-Gomez, Eduardo:
Ctr Med Nacl 20 Noviembre, ISSSTE, Lab Expt Metab & Clin Res, Mexico City, DF, Mexico
Gomez-Calderon, Alan De Jesus:
Ctr Med Nacl 20 Noviembre, ISSSTE, Tissue Engn & Regenerat Med Res Grp, Mexico City, DF, Mexico
Ctr Med Nacl 20 Noviembre, ISSSTE, Coordinac Invest, Mexico City, DF, Mexico
Tellez-Gonzalez, Mario Antonio:
Ctr Med Nacl 20 Noviembre, ISSSTE, Tissue Engn & Regenerat Med Res Grp, Mexico City, DF, Mexico
Ctr Med Nacl 20 Noviembre, ISSSTE, Coordinac Invest, Mexico City, DF, Mexico
Mondragon-Teran, Paul:
Ctr Med Nacl 20 Noviembre, ISSSTE, Tissue Engn & Regenerat Med Res Grp, Mexico City, DF, Mexico
Ctr Med Nacl 20 Noviembre, ISSSTE, Coordinac Invest, Mexico City, DF, Mexico
|