Enhancing rotifer Brachionus calyciflorus population growth with commercial probiotics
Por:
Alonso Contreras-Tapia, Ruben, Elena Castellanos-Paez, Maria, Nandini, S., Ivonne Benitez-Diaz-Miron, Marcela, Garza-Mourino, Gabriela
Publicada:
1 may 2020
Categoría:
Aquatic science
Resumen:
The effect of a commercial probiotic (NanoCrusta, Altacrusta, Mexico
City, Mexico) on the growth performance of Brachionus calyciflorus
Pallas, 1766, was evaluated. In a first approach, probiotics were
supplied in four densities (2.0 x 10(3), 1.1 x 10(5), 2.1 x 10(5) and
2.1 x 10(6 )cells/ml), alone and in combination with Chlorella vulgaris
(1 x 10(6 )cells/ml). The test rotifer did not grow on the probiotic
alone. However, when probiotics + C. vulgaris were added, the maximum
densities (D-max; ind/ml) and population growth rates (r) observed were
higher. In the second experiment, probiotics were supplied at five
higher densities (2.1 x 10(6), 4.2 x 10(6), 8.5 x 10(6), 1.7 x 10(7) and
3.4 x 10(7 )cells/ml) with C. vulgaris and a control treatment with only
C. vulgaris (probiotic-free). Treatments supplied with probiotics
between 2.1 x 10(6) and 1.7 x 10(7 )cells/ml showed significantly higher
D-max and r than the control treatment. The results showed a positive
effect of probiotic bacteria when supplied with C. vulgaris. The best
outcome showed a D-max 2.16 times and an r 1.63 times higher than the
density of the control treatment. Growth rates were higher in the
treatments with probiotics compared to the control. We conclude that
application of NanoCrusta is feasible to improve B. calyciflorus
production, but the effects need to be tested in larger scales.
Filiaciones:
Alonso Contreras-Tapia, Ruben:
Univ Autonoma Metropolitana, Programa Doctorado Ciencias Biol & Salud, Unidad Xochimilco, Ciudad De Mexico, Mexico
Univ Autonoma Metropolitana, Lab Rotiferol & Biol Mol Plancton, Dept El Hombre & Ambiente, Unidad Xochimilco, Ciudad De Mexico, Mexico
Elena Castellanos-Paez, Maria:
Univ Autonoma Metropolitana, Lab Rotiferol & Biol Mol Plancton, Dept El Hombre & Ambiente, Unidad Xochimilco, Ciudad De Mexico, Mexico
Nandini, S.:
Univ Nacl Autonoma Mexico, Lab Zool Acuat, FES Iztacala, Tlalnepantla De Baz, Estado De Mexic, Mexico
Ivonne Benitez-Diaz-Miron, Marcela:
Univ Autonoma Metropolitana, Lab Rotiferol & Biol Mol Plancton, Dept El Hombre & Ambiente, Unidad Xochimilco, Ciudad De Mexico, Mexico
Garza-Mourino, Gabriela:
Univ Autonoma Metropolitana, Lab Rotiferol & Biol Mol Plancton, Dept El Hombre & Ambiente, Unidad Xochimilco, Ciudad De Mexico, Mexico
|