Participation of Extracellular Vesicles from Zika-Virus-Infected Mosquito Cells in the Modification of Naive Cells' Behavior by Mediating Cell-to-Cell Transmission of Viral Elements
Por:
Pablo Martinez-Rojas, Pedro, Quiroz-Garcia, Elizabeth, Monroy-Martinez, Veronica, Teresa Agredano-Moreno, Lourdes, Felipe Jimenez-Garcia, Luis, Ruiz-Ordaz, Blanca H.
Publicada:
1 ene 2020
Categoría:
Biochemistry, genetics and molecular biology (miscellaneous)
Resumen:
To date, no safe vaccine or antivirals for Zika virus (ZIKV) infection
have been found. The pathogenesis of severe Zika, where host and viral
factors participate, remains unclear. For the control of Zika, it is
important to understand how ZIKV interacts with different host cells.
Knowledge of the targeted cellular pathways which allow ZIKV to
productively replicate and/or establish prolonged viral persistence
contributes to novel vaccines and therapies. Monocytes and endothelial
vascular cells are the main ZIKV targets. During the infection process,
cells are capable of releasing extracellular vesicles (EVs). EVs are
mediators of intercellular communication. We found that mosquito EVs
released from ZIKV-infected (C6/36) cells carry viral RNA and ZIKV-E
protein and are able to infect and activate naive mosquito and mammalian
cells. ZIKV C6/36 EVs promote the differentiation of naive monocytes and
induce a pro-inflammatory state with tumor necrosis factor-alpha
(TNF-alpha) mRNA expression. ZIKV C6/36 EVs participate in endothelial
vascular cell damage by inducing coagulation (TF) and inflammation
(PAR-1) receptors at the endothelial surface of the cell membranes and
promote a pro-inflammatory state with increased endothelial
permeability. These data suggest that ZIKV C6/36 EVs may contribute to
the pathogenesis of ZIKV infection in human hosts.
Filiaciones:
Pablo Martinez-Rojas, Pedro:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Av Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Quiroz-Garcia, Elizabeth:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Av Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Monroy-Martinez, Veronica:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Av Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Teresa Agredano-Moreno, Lourdes:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Av Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Felipe Jimenez-Garcia, Luis:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Av Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Ruiz-Ordaz, Blanca H.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Av Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
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