Ibuprofen at environmentally relevant concentrations alters embryonic development, induces teratogenesis and oxidative stress in Cyprinus carpio
Por:
Margarita Gutierrez-Noya, Veronica, Manuel Gomez-Olivan, Leobardo, del Carmen Ramirez-Montero, Maria, Islas-Flores, Hariz, Galar-Martinez, Marcela, Dublan-Garcia, Octavio, Romero, Rubi
Publicada:
25 mar 2020
Resumen:
Ibuprofen (IBU) is a non-steroidal anti-inflammatory (NSAIDs) that is
used in various conditions. The prescriptions and the global consumption
of this drug are very high and its annual production oscillates in
millions of tons, this generates that the IBU is present in many
waterbodies because it is discharged through the municipal, hospital and
industrial effluents. For the above, the purpose of this work was to
determine if IBU at environmentally relevant concentrations was capable
of inducing alterations to embryonic development, teratogenic effects
and oxidative stress in oocytes and embryos of Cyprinus carpio. Oocytes
of common carp were exposed to IBU concentrations between 1.5 and 115 mu
g L-1 (environmentally relevant). LC50 and EC50 of malformations were
determined to calculate the teratogenic index (Tl). Also, main
alterations to embryonic development and teratogenic effects were
evaluated. Oxidative stress was evaluated by determining biomarkers of
cellular oxidation and a ntiox idation using the same concentrations at
72 and 96 hpf in embryos of Cyprinus airplane results showed a LC50 of
4.17 mu g L-1, EC50 of 139 mu g L-1 and Tl of 3.0. The main embryonic
development disorders and teratogenic effects were delayed hatching,
hypopigmentation, pericardial edema, yolk deformation, and developmental
delay. Biomarkers of cellular oxidation and antioxidants were increased
with respect to the control in a concentration-dependent manner. The
results of the study allow us to conclude that IBU at environmentally
relevant concentrations is capable of inducing embryotoxicity and
teratogenicity in a fish of commercial interest like Cyprinus carpio.
(C) 2019 Elsevier B.V. All rights reserved.
Filiaciones:
Margarita Gutierrez-Noya, Veronica:
Univ Autonoma Estado Mexico, Fac Quim, Lab Toxicol Ambiental, Paseo Colon Intersecc Paseo Tollocan, Toluca 50120, Estado De Mexic, Mexico
Manuel Gomez-Olivan, Leobardo:
Univ Autonoma Estado Mexico, Fac Quim, Lab Toxicol Ambiental, Paseo Colon Intersecc Paseo Tollocan, Toluca 50120, Estado De Mexic, Mexico
del Carmen Ramirez-Montero, Maria:
Univ Autonoma Estado Mexico, Fac Quim, Lab Toxicol Ambiental, Paseo Colon Intersecc Paseo Tollocan, Toluca 50120, Estado De Mexic, Mexico
Islas-Flores, Hariz:
Univ Autonoma Estado Mexico, Fac Quim, Lab Toxicol Ambiental, Paseo Colon Intersecc Paseo Tollocan, Toluca 50120, Estado De Mexic, Mexico
Galar-Martinez, Marcela:
Inst Politecn Nacl, Dept Farm, Lab Toxicol Acuat, Unidad Profes Adolfo Lopez Mateos,Escuela Nacl Ci, Av Wilfrido Massieu S-N & Cerrada Manuel Stamp, Ciudad De Mexico 07700, Mexico
Dublan-Garcia, Octavio:
Univ Autonoma Estado Mexico, Fac Quim, Lab Toxicol Ambiental, Paseo Colon Intersecc Paseo Tollocan, Toluca 50120, Estado De Mexic, Mexico
Romero, Rubi:
Univ Autonoma Estado Mexico, Ctr Conjunto Invest Quim Sustentable, UAEM UNAM, Km 14-5 Carretera Toluca Atlacomulco, Toluca 50200, Mexico
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