Signature patterns in region V4 of small ruminant lentivirus surface protein in sheep and goats
Por:
Gonzalez Mendez, Ana Silvia, Ceron Tellez, Fernando, Tortora Perez, Jorge Luis, Martinez Rodriguez, Humberto Alejandro, Garcia Flores, Maria Martha, Ramirez Alvarez, Hugo
Publicada:
1 ene 2020
Resumen:
The env gene in Small Ruminant Lentiviruses (SRLV) encodes the surface glycoprotein (SU) that divides into conserved (C1-C4) and variable regions (V1-V5). SRLV region V4 has been found to be homologous to the V3 region of human lentivirus (HIV). HIV V3 is responsible for tropism and the development of nervous clinical patterns when there is a tendency to conserve amino acids in specific “signature pattern” positions. The goal of this study was to identify signature patterns in the V4 region of the SU, which is encoded by the SRLV env gene. Secondarily, to understand how these signature patterns are associated with different clinical status in naturally infected sheep and goats. Starting with 244 samples from seropositive animals from nine Mexican states, we amplified the V4 region using nested PCR and obtained 49 SRLV sequences from peripheral blood leukocytes. Based on phylogenetic analysis results, we identified three groups: asymptomatic genotypes A (Ssx GA) and B (Ssx GB), as well as animals with arthritic presentation, genotype B (A GB). Similarity levels between group sequences ranged from 67.9%–86.7%, with a genetic diversity ranging from 12.7%–29.5% and a dN / dS ratio that indicated negative selection. Analyses using Vespa and Entropy programs identified four residues at positions 54, 78, 79 and 82 in SU region V4 as possible signature patterns, although with variable statistical significance. However, position 54 residues “N” (p = 0.017), “T” (p = 0.001) and “G” (p = 0.024) in groups A GB, Ssx GA and Ssx GB respectively, best characterized the signature patterns. The results obtained identified a signature pattern related to different genotypes and clinical status by SRLV in sheep and goats. © 2020 Elsevier B.V.
Filiaciones:
Gonzalez Mendez, Ana Silvia:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlan Izcalli, Estado de México C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Fac Higher Educ Cuautitlan Vet Med, Virol Genet & Mol Biol Lab, Campus 4, Cuautitlan Izcaili 54714, Estado De Mexic, Mexico
Ceron Tellez, Fernando:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlan Izcalli, Estado de México C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Fac Higher Educ Cuautitlan Vet Med, Virol Genet & Mol Biol Lab, Campus 4, Cuautitlan Izcaili 54714, Estado De Mexic, Mexico
Tortora Perez, Jorge Luis:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlan Izcalli, Estado de México C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Fac Higher Educ Cuautitlan Vet Med, Virol Genet & Mol Biol Lab, Campus 4, Cuautitlan Izcaili 54714, Estado De Mexic, Mexico
Martinez Rodriguez, Humberto Alejandro:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlan Izcalli, Estado de México C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Fac Higher Educ Cuautitlan Vet Med, Virol Genet & Mol Biol Lab, Campus 4, Cuautitlan Izcaili 54714, Estado De Mexic, Mexico
Garcia Flores, Maria Martha:
Laboratory of Immunovirology, Medical Research in Immunology Unit, Pediatric Hospital, National Medical Center XXI Century, Mexican Institute of Social Security, Mexico
Mexican Inst Social Secur, Natl Med Ctr XXI Century, Pediat Hosp, Lab Immunovirol,Med Res Immunol Unit, Mexico City, DF, Mexico
Ramirez Alvarez, Hugo:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlan Izcalli, Estado de México C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Fac Higher Educ Cuautitlan Vet Med, Virol Genet & Mol Biol Lab, Campus 4, Cuautitlan Izcaili 54714, Estado De Mexic, Mexico
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