Lack of association between amino acid sequences of the bovine leukemia virus envelope and varying stages of infection in dairy cattle
Por:
Ceron Tellez, Fernando, Gonzalez Mendez, Ana Silvia, Tortora Perez, Jorge Luis, Loza-Rubio, Elizabeth, Ramirez Alvarez, Hugo
Publicada:
1 ene 2020
Resumen:
We collected 724 blood samples from dairy cattle from six Mexican states, and tested them for the presence of antibodies against BLV using a commercial ELISA test. Our study groups consisted of 32 samples: 12 asymptomatic cows, 12 cows with lymphocytosis and 8 samples of tumor tissue of the abomasum and heart of cattle with lymphoma. We designed three pairs of primers to amplify the complete BLV env gene, and obtained a fragment of 1548 nucleotides in length with the sequenced products. According to the phylogenetic tree we constructed to identify the viral genotype, 96.87 % of the sequences grouped into genotype 1, while a single sample from a cow with lymphocytosis (3.13 %) was associated with genotype 3 sequences. The similarity between the Mexican BLV sequences ranged from 0.985–1.00. In addition, the proportion of non-synonymous and synonymous mutations indicated negative selection. We did not identify any conserved residues in the viral protein sequences that could be related to BLV infection stage in cattle. Proviral quantification was performed using quantitative polymerase chain reaction, and we used Mood´s median test as statistical analysis. We found no significant association between proviral load and phase of infection. The sequences showed high similarity without any association between BLV surface glycoprotein and the different infection stages, nor differences in the proviral load. BLV genotype 1 was identified as prevalent in the studied samples, and for the first time in Mexico, we identified BLV genotype 3 in cattle. © 2020 Elsevier B.V.
Filiaciones:
Ceron Tellez, Fernando:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlán Izcalli Estado de México, C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Virol Genet & Mol Biol Lab, Fac Higher Educ, Cuaulitlan,Vet Med, Km 2-5 Carretera Cuautitlan Teoloyucan San Sebast, Cuautitilan Izcalli 54714, Estado De Mexic, Mexico
Gonzalez Mendez, Ana Silvia:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlán Izcalli Estado de México, C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Virol Genet & Mol Biol Lab, Fac Higher Educ, Cuaulitlan,Vet Med, Km 2-5 Carretera Cuautitlan Teoloyucan San Sebast, Cuautitilan Izcalli 54714, Estado De Mexic, Mexico
Tortora Perez, Jorge Luis:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlán Izcalli Estado de México, C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Virol Genet & Mol Biol Lab, Fac Higher Educ, Cuaulitlan,Vet Med, Km 2-5 Carretera Cuautitlan Teoloyucan San Sebast, Cuautitilan Izcalli 54714, Estado De Mexic, Mexico
Loza-Rubio, Elizabeth:
National Center of Research in Animal Microbiology and Innocuity, INIFAP, Mexico City, CP. 05110, Mexico
INIFAP, Natl Ctr Res Anim Microbiol & Innocu, Mexico City 05110, DF, Mexico
Ramirez Alvarez, Hugo:
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km. 2.5 Carretera Cuautitlán-Teoloyucan San Sebastián Xhala, Cuautitlán Izcalli Estado de México, C.P. 54714, Mexico
Univ Nacl Autonoma Mexico, Virol Genet & Mol Biol Lab, Fac Higher Educ, Cuaulitlan,Vet Med, Km 2-5 Carretera Cuautitlan Teoloyucan San Sebast, Cuautitilan Izcalli 54714, Estado De Mexic, Mexico
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