The NR3C1 gene expression is a potential surrogate biomarker for risk and diagnosis of posttraumatic stress disorder.
Por:
Gonzalez Ramirez, Claudia, Villavicencio Queijeiro, Alexa, Jimenez Morales, Silvia, Barcenas Lopez, Diego, Hidalgo Miranda, Alfredo, Ruiz Chow, Angel, Tellez Cardenas, Liliana, Guardado Estrada, Mariano
Publicada:
1 ene 2020
Resumen:
Posttraumatic Stress Disorder (PTSD) is an anxiety disorder which occurs after a traumatic event. The NR3C1 gene codes for the Glucocorticoid Receptor, which participate in the Hypothalamic-Pituitary-Adrenal (HPA) axis and is altered in PTSD patients. To evaluate whether the NR3C1 gene expression in peripheral blood could be useful as a diagnosis biomarker, a total of 32 PTSD patients and 59 healthy controls were analyzed with quantitative RT-PCR. Also, to assess if NR3C1 dysregulation is associated with hypocortisolism in PTSD patients, serum cortisol was quantified by ELISA in a subset of these samples. Significant NR3C1 over-expression was found in PTSD patients compared with controls, and this was higher in patients with acute PTSD. The Area Under the Curve (AUC) of NR3C1 gene expression was 0.797. The sensibility and specificity of NRC1 gene expression to diagnose PTSD was 62.5% and 89.8%, respectively. We also found that an up-regulation of NR3C1 increased the risk for being diagnosed with PTSD (OR= 12.8, 95%, CI 4-41.4). Finally, the NR3C1 gene expression was inversely related with serum cortisol in PTSD patients. The present results suggest that NR3C1 gene expression could be a promising biomarker for PTSD diagnosis and estimate the risk for disease development. © 2020 Elsevier B.V.
Filiaciones:
Gonzalez Ramirez, Claudia:
Laboratorio de Genética de la Licenciatura en Ciencia Forense, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Lab Genet Licenciatura Ciencia Forense, Mexico City, DF, Mexico
Villavicencio Queijeiro, Alexa:
Laboratorio de Genética de la Licenciatura en Ciencia Forense, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Lab Genet Licenciatura Ciencia Forense, Mexico City, DF, Mexico
Jimenez Morales, Silvia:
Laboratorio Genómica del Cáncer, Instituto Nacional de Medicina Genómica, Mexico
Inst Nacl Med Genom, Lab Genom Canc, Ciudad De Mexico, Cdmx, Mexico
Barcenas Lopez, Diego:
Laboratorio Genómica del Cáncer, Instituto Nacional de Medicina Genómica, Mexico
Inst Nacl Med Genom, Lab Genom Canc, Ciudad De Mexico, Cdmx, Mexico
Hidalgo Miranda, Alfredo:
Laboratorio Genómica del Cáncer, Instituto Nacional de Medicina Genómica, Mexico
Inst Nacl Med Genom, Lab Genom Canc, Ciudad De Mexico, Cdmx, Mexico
Ruiz Chow, Angel:
Instituto Nacional de Neurología y Neurocirugía “Manuel Velasco Suárez”, Mexico
Inst Nacl Neurol & Neurocirugia Manuel Velasco Su, Mexico City, DF, Mexico
Tellez Cardenas, Liliana:
Medicina Ocupacional, Secretaría de Marina, Mexico
Secretaria Marina, Med Ocupac, Veracruz, Mexico
Guardado Estrada, Mariano:
Laboratorio de Genética de la Licenciatura en Ciencia Forense, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Lab Genet Licenciatura Ciencia Forense, Mexico City, DF, Mexico
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