The BPtpA protein from Burkholderia cenocepacia belongs to a new subclass of low molecular weight protein tyrosine phosphatases
Por:
Sánchez-Rodríguez R., González G.M., Becerril-García M.A., Treviño-Rangel R.D.J., Marcos-Vilchis A., González-Pedrajo B., Valvano M.A., Andrade A.
Publicada:
1 ene 2020
Resumen:
Low molecular weight protein tyrosine phosphatases (LMW-PTP) are ubiquitous enzymes found across a spectrum of genera from prokaryotes to higher eukaryotes. LMW-PTP belong to the Cys-based PTP class II protein family. Here, we show that LMW-PTP can be categorized into two different groups, referred as class II subdivision I (class II.I) and subdivision II (class II.II). Using BPtpA from the opportunistic pathogen Burkholderia cenocepacia, as a representative member of the LMW-PTP class II.I, we demonstrated that four conserved residues (W47, H48, D80, and F81) are required for enzyme function. Guided by an in silico model of BPtpA, we show that the conserved residues at a3-helix (D80 and F81) contribute to protein stability, while the other conserved residues in the W-loop (W47 and H48) likely play a role in substrate recognition. Overall, our results provide new information on LMW-PTP protein family and establish B. cenocepacia as a suitable model to investigate how substrates are recognized and sorted by these proteins. © 2020 Elsevier Inc.
Filiaciones:
Sánchez-Rodríguez R.:
Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Microbiología. MonterreyNuevo León 64460, Mexico
Univ Autonoma Nuevo Leon, Fac Med, Dept Microbiol, Av Francisco I Madero S-N, Monterrey 64460, Nuevo Leon, Mexico
González G.M.:
Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Microbiología. MonterreyNuevo León 64460, Mexico
Univ Autonoma Nuevo Leon, Fac Med, Dept Microbiol, Av Francisco I Madero S-N, Monterrey 64460, Nuevo Leon, Mexico
Becerril-García M.A.:
Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Microbiología. MonterreyNuevo León 64460, Mexico
Univ Autonoma Nuevo Leon, Fac Med, Dept Microbiol, Av Francisco I Madero S-N, Monterrey 64460, Nuevo Leon, Mexico
Treviño-Rangel R.D.J.:
Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Microbiología. MonterreyNuevo León 64460, Mexico
Marcos-Vilchis A.:
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, 70-243, Mexico
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Genet Mol, Mexico City 70243, DF, Mexico
González-Pedrajo B.:
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, 70-243, Mexico
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Genet Mol, Mexico City 70243, DF, Mexico
Valvano M.A.:
Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, BT9 7BL, United Kingdom
Queens Univ Belfast, Wellcome Wolfson Inst Expt Med, Belfast BT9 7BL, Antrim, North Ireland
Andrade A.:
Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Microbiología. MonterreyNuevo León 64460, Mexico
Univ Autonoma Nuevo Leon, Fac Med, Dept Microbiol, Av Francisco I Madero S-N, Monterrey 64460, Nuevo Leon, Mexico
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