COMPARISON OF THE FECAL BACTERIAL MICROBIOTA COMPOSITION BETWEEN WILD AND CAPTIVE BOLSON TORTOISES (GOPHERUS FLAVOMARGINATUS)
Por:
Garcia-De la Pena, Cristina, Garduno-Nino, Estefania, Vaca-Paniagua, Felipe, Diaz-Velasquez, Clara, Barrows, Cameron W., Gomez-Gil, Bruno, Manuel Valenzuela-Nunez, Luis
Publicada:
1 dic 2019
Resumen:
Research analyzing fecal microbiota provides important insights into the
biological mechanisms affecting the health of animals. The Bolson
Tortoise (Gopherus flavonsarginatus) is endemic to northern Mexico and
is threatened mainly by habitat loss and collecting by humans. Here, we
characterized the fecal bacterial communities of wild (10 samples) and
captive (10 samples) G. flavomarginatus, and compared their microbiota
analyzing the V3-V4 region of 16S rRNA gene using high-throughput
sequencing technology on Illumina Miseq platform. We identified 21
phyla, 44 classes, 66 orders, 139 families, and 463 genera for all G.
flavoinarg,intrIns fecal samples. Bacterial communities showed
significant differences between wild and captive tortoises at each
taxonomical level. Firmicutes and Cyanobacteria were the most
predominant phyla in wild tortoises. In captive tortoises, Firmicutes
was the dominant phylum, followed by Fibrobacteres. At the genus level,
Hydrogens:pima, GQ422712_g, and tleetivibrio were significantly more
abundant in the wild population, whereas LDWW_g, HQ716403_g,
AB239481_g, and Sporobacter predominated in the captive population.
Diet could be the main factor responsible for differences between fecal
microbiotas. All the individuals we analyzed were clinically healthy,
which would mean that their bacterial communities are in apparent
equilibrium. Nevertheless, the differences between the fecal bacteria of
wild and captive tortoise populations indicate that, prior to releasing
captive animals into the wild, inoculating them with wild-tortoise
bacteria may improve their success at adjusting to a wild diet.
Filiaciones:
Garcia-De la Pena, Cristina:
Univ Juarez Estado Durango, Fac Ciencias Biol, Durango 35010, Mexico
Garduno-Nino, Estefania:
Univ Juarez Estado Durango, Fac Ciencias Biol, Durango 35010, Mexico
Vaca-Paniagua, Felipe:
Fac Estudios Super Iztacala, Lab Nacl Salud Diagnost Mol & Efecto Ambiental, Tlalnepantla 54090, Estado De Mexic, Mexico
Inst Nacl Cancerol, Ciudad De Mexico 14080, Mexico
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Tlalnepantla 54090, Estado De Mexic, Mexico
Diaz-Velasquez, Clara:
Fac Estudios Super Iztacala, Lab Nacl Salud Diagnost Mol & Efecto Ambiental, Tlalnepantla 54090, Estado De Mexic, Mexico
Barrows, Cameron W.:
Univ Calif Riverside, Ctr Conservat Biol, Palm Desert, CA 92211 USA
Gomez-Gil, Bruno:
CIAD, Unidad Mazatlan Acuicultura & Manejo Ambiental, Mazatlan 82100, Sinaloa, Mexico
Manuel Valenzuela-Nunez, Luis:
Univ Juarez Estado Durango, Fac Ciencias Biol, Durango 35010, Mexico
|