Mangiferin-loaded polymeric nanoparticles: Optical characterization, effect of antitopoisomerase I, and cytotoxicity
Por:
Razura-Carmona F.F., Perez-Larios, Alejandro, Gonzalez-Silva, Napoleon, Herrera-Martinez, Mayra, Medina-Torres, Luis, Sáyago-Ayerdi S.G., Sánchez-Burgos J.A.
Publicada:
1 ene 2019
Resumen:
Mangiferin is an important xanthone compound presenting various biological activities. The objective of this study was to develop, characterize physicochemical properties, and evaluate the anti-topoisomerase activity of poly(lactic-co-glycolic acid) (PLGA) nanoparticles containing mangiferin. The nanoparticles were developed by the emulsion solvent evaporation method and the optimal formulation was obtained with a response surface methodology (RSM); this formulation showed a mean size of 176.7 ± 1.021 nm with a 0.153 polydispersibility index (PDI) value, and mangiferin encapsulation efficiency was about 55%. The optimal conditions (6000 rpm, 10 min, and 300 µg of mangiferin) obtained 77% and the highest entrapment efficiency (97%). The in vitro release profile demonstrated a gradual release of mangiferin from 15 to 180 min in acidic conditions (pH 1.5). The fingerprint showed a modification in the maximum absorption wavelength of both the polymer and the mangiferin. Results of anti-toposiomerase assay showed that the optimal formulation (MG4, 25 µg/mL) had antiproliferative activity. High concentrations (2500 µg/mL) of MG4 showed non-in vitro cytotoxic effect on BEAS 2B and HEPG2. Finally, this study showed an encapsulation process with in vitro gastric digestion resistance (1.5 h) and without interfering with the metabolism of healthy cells and their biological activity. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Razura-Carmona F.F.:
Tecnológico Nacional de México/I.T. Tepic, Laboratorio Integran de Investigación en Alimentos, Lagos del Country, Tepic, Nayarit CP 63175, Mexico
Perez-Larios, Alejandro:
Division of Agricultural Sciences and Engineering, University Center of the Altos, University of Guadalajara, Tepatitlán de Morelos, Jalisco CP 47620, Mexico
Univ Guadalajara, Univ Ctr Altos, Div Agr Sci & Engn, Tepatitlan De Morelos Cp 47620, Jalisco, Mexico
Gonzalez-Silva, Napoleon:
Division of Agricultural Sciences and Engineering, University Center of the Altos, University of Guadalajara, Tepatitlán de Morelos, Jalisco CP 47620, Mexico
Univ Guadalajara, Univ Ctr Altos, Div Agr Sci & Engn, Tepatitlan De Morelos Cp 47620, Jalisco, Mexico
Herrera-Martinez, Mayra:
Instituto de Farmacobiología, Universidad de la Cañada, Teotitlán de Flores Magón, Oaxaca CP 68540, Mexico
Univ Canada, Inst Farmacobiol, Teotitlan De Flores Mago 68540, Oaxaca, Mexico
Medina-Torres, Luis:
Facultad de Química, Universidad Nacional Autónoma de México, México D.F., CP 04510, Mexico
Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico
Sáyago-Ayerdi S.G.:
Tecnológico Nacional de México/I.T. Tepic, Laboratorio Integran de Investigación en Alimentos, Lagos del Country, Tepic, Nayarit CP 63175, Mexico
Sánchez-Burgos J.A.:
Tecnológico Nacional de México/I.T. Tepic, Laboratorio Integran de Investigación en Alimentos, Lagos del Country, Tepic, Nayarit CP 63175, Mexico
Tecnol Nacl Mexico IT Tepic, Lab Integran Invest Alimentos, Tepic 63175, Nayarit, Mexico
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