Use of next-generation amplicon sequencing to study Blastocystis genetic diversity in a rural human population from Mexico
Por:
Rojas-Velazquez, Liliana, Maloney, Jenny G., Molokin, Aleksey, Moran, Patricia, Serrano-Vazquez, Angelica, Gonzalez, Enrique, Perez-Juarez, Horacio, Ximenez, Cecilia, Santin, Monica
Publicada:
27 nov 2019
Resumen:
Background The intestinal parasite Blastocystis is found in humans and
animals around the world. It is spread through the consumption of
contaminated food and water and has been associated with a variety of
intestinal symptoms. Blastocystis is one of the most common intestinal
parasites in humans, yet its prevalence and distribution in humans in
North America is not well characterized. Methods Next-generation
amplicon sequencing of a region of the Blastocystis SSU rRNA gene was
applied to DNA extracted from fecal specimens obtained from 182
inhabitants of a rural population in Mexico to characterize Blastocystis
prevalence, subtype distribution, and intra-host subtype diversity in
humans. Results Of the 182 samples tested in this study, 68.1% (124)
contained one or more Blastocystis subtypes. Subtype 3 was the most
common subtype observed and was found in 81.5% of the positive samples.
Subtype 1, 16.9% of the positive samples, and subtype 2, 17.7% of the
positive samples, were also found in this population. Mixed infections
were observed in 13.7% of the positive samples. In this population, the
odds of having Blastocystis increased in adulthood (> 15 years; OR:
1.72, P < 0.0001), and the odds of having subtype 1 increased in the
presence of farm animals (OR: 1.51, P = 0.03). The odds of having
subtype 1, subtype 2, or a mixed infection decreased in the presence of
cement flooring (OR: - 1.61, P = 0.005; OR: - 1.14, P = 0.03; OR: -
1.48, P = 0.02) possibly indicating socioeconomic factors are involved
in the risk of acquiring one of these subtypes. Conclusions These data
contribute to our understanding of the epidemiology of Blastocystis
infection in humans and can be used to shape future studies which aim to
better characterize the transmission pathways and health outcomes of
Blastocystis infections.
Filiaciones:
Rojas-Velazquez, Liliana:
Univ Nacl Autonoma Mexico, Unidad Invest Med Expt, Fac Med, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Unidad Posgrad, Mexico City, DF, Mexico
Maloney, Jenny G.:
ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD USA
Molokin, Aleksey:
ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD USA
Moran, Patricia:
Univ Nacl Autonoma Mexico, Unidad Invest Med Expt, Fac Med, Mexico City, DF, Mexico
Serrano-Vazquez, Angelica:
Univ Nacl Autonoma Mexico, Unidad Invest Med Expt, Fac Med, Mexico City, DF, Mexico
Gonzalez, Enrique:
Univ Nacl Autonoma Mexico, Unidad Invest Med Expt, Fac Med, Mexico City, DF, Mexico
Perez-Juarez, Horacio:
Univ Nacl Autonoma Mexico, Unidad Invest Med Expt, Fac Med, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Unidad Posgrad, Mexico City, DF, Mexico
Ximenez, Cecilia:
Univ Nacl Autonoma Mexico, Unidad Invest Med Expt, Fac Med, Mexico City, DF, Mexico
Santin, Monica:
ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD USA
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