Mesenchymal Stromal Cells from the Epidermis and Dermis of Psoriasis Patients: Morphology, Immunophenotype, Differentiation Patterns, and Regulation of T Cell Proliferation
Por:
Castro-Manrreza, M. E., Bonifaz, L., Castro-Escamilla, O., Monroy-Garcia, A., Cortes-Morales, A., Hernandez-Estevez, E., Hernandez-Cristino, J., Mayani, H., Montesinos, J. J.
Publicada:
1 dic 2019
Resumen:
Psoriasis is a skin disease characterized by hyperproliferation of
keratinocytes and chronic inflammation. Mesenchymal stem/stromal cells
(MSCs) exhibit an immunoregulatory function that can be altered in the
skin of these patients. However, to date, the presence and functional
capacity of MSCs in the dermis and epidermis of patients with psoriasis
have not been fully established. In the present study, we evaluated the
presence of MSCs in the skin of patients by obtaining adherent cells
from the dermis and epidermis of lesional and nonlesional areas and
characterizing them in a comparative manner with corresponding cells
obtained from the dermis (HD-MSCs) and epidermis (HE-MSCs) of healthy
donors. We determined whether the adherent cells had immunophenotypic
profiles and differentiation potentials that were characteristic of
MSCs. In addition, we analyzed their immunosuppression function by
evaluating their capacity to decrease T cell proliferation. Our results
indicate the presence of MSCs in the dermis and epidermis of healthy
donors and patients with psoriasis; adherent cells from all skin sources
exhibited MSC characteristics, such as expression of CD73, CD90, and
CD105 markers and a lack of hematopoietic and endothelial marker
expression. However, the cell populations obtained showed differences in
differentiation potential toward adipogenic, osteogenic, and
chondrogenic lineages. In addition, we observed a low MSC obtention
frequency in nonlesional epidermal samples (NLE-MSCs), which also showed
alterations in morphology and proliferation rate. Interestingly, MSCs
from both the nonlesional dermis (NLD-MSCs) and lesional dermis
(LD-MSCs) showed higher HLA class I antigen (HLA-I) expression than
HD-MSCs. Moreover, NLD-MSCs showed a low T cell proliferation
suppression capacity. In summary, this study demonstrates the presence
of MSCs in the epidermis and dermis of patients with psoriasis and
suggests that such cells may favor the inflammatory process and thus
psoriatic lesion development through high HLA-I expression and low
immunosuppression capacity.
Filiaciones:
Castro-Manrreza, M. E.:
Univ Nacl Autonoma Mexico, Lab Inmunol & Celulas Troncales, FES Zaragoza, Unidad Multidisciplinaria Invest Expt Zaragoza, Mexico City, DF, Mexico
Bonifaz, L.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Hosp Especialidades, Unidad Invest Med Inmunoquim, Mexico City, DF, Mexico
Castro-Escamilla, O.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Hosp Especialidades, Unidad Invest Med Inmunoquim, Mexico City, DF, Mexico
Monroy-Garcia, A.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Lab Inmunol & Canc, Unidad Invest Med Enfermedades Oncol, Mexico City, DF, Mexico
Cortes-Morales, A.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Lab Celulas Troncales Mesenquimales, Unidad Invest Med Enfermedades Oncol, Mexico City, DF, Mexico
Hernandez-Estevez, E.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Lab Celulas Troncales Mesenquimales, Unidad Invest Med Enfermedades Oncol, Mexico City, DF, Mexico
Hernandez-Cristino, J.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Lab Celulas Troncales Mesenquimales, Unidad Invest Med Enfermedades Oncol, Mexico City, DF, Mexico
Mayani, H.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Lab Celulas Troncales Hematopoyet, Unidad Invest Med Enfermedades Oncol, Mexico City, DF, Mexico
Montesinos, J. J.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Lab Celulas Troncales Mesenquimales, Unidad Invest Med Enfermedades Oncol, Mexico City, DF, Mexico
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