Unpacking the aggregation-oligomerization-fibrillization process of naturally-occurring hIAPP amyloid oligomers isolated directly from sera of children with obesity or diabetes mellitus
Por:
Altamirano-Bustamante, Myriam M., Altamirano-Bustamante, Nelly F., Larralde-Laborde, Mateo, Lara-Martinez, Reyna, Leyva-Garcia, Edgar, Garrido-Magana, Eulalia, Rojas, Gerardo, Felipe Jimenez-Garcia, Luis, Revilla-Monsalve, Cristina, Altamirano, Perla, Calzada-Leon, Raul
Publicada:
5 dic 2019
Categoría:
Multidisciplinary
Resumen:
The formation of amyloid oligomers and fibrils of the human islet
amyloid polypeptide (hIAPP) has been linked with beta- cell failure and
death which causes the onset, progression, and comorbidities of
diabetes. We begin to unpack the
aggregation-oligomerization-fibrillization process of these oligomers
taken from sera of pediatric patients. The naturally occurring or real
hIAPP (not synthetic) amyloid oligomers (RIAO) were successfully
isolated, we demonstrated the presence of homo (dodecamers, hexamers,
and trimers) and hetero-RIAO, as well as several biophysical
characterizations which allow us to learn from the real phenomenon
taking place. We found that the aggregation/oligomerization process is
active in the sera and showed that it happens very fast. The RIAO can
form fibers and react with anti-hIAPP and anti-amyloid oligomers
antibodies. Our results opens the epistemic horizon and reveal real
differences between the four groups (Controls vs obesity, T1DM or T2DM)
accelerating the process of understanding and discovering novel and more
efficient prevention, diagnostic, transmission and therapeutic pathways.
Filiaciones:
Altamirano-Bustamante, Myriam M.:
Ctr Med Nacl Siglo XXI, Inst Mexicano Seguro Social, Unidad Invest Enfermedades Metabol, Mexico City, DF, Mexico
Unidad de Investigación en Enfermedades Metabólicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Altamirano-Bustamante, Nelly F.:
Inst Nacl Pediat, Mexico City, DF, Mexico
Instituto Nacional de Pediatría, México City, Mexico
Larralde-Laborde, Mateo:
Ctr Med Nacl Siglo XXI, Inst Mexicano Seguro Social, Unidad Invest Enfermedades Metabol, Mexico City, DF, Mexico
Unidad de Investigación en Enfermedades Metabólicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Lara-Martinez, Reyna:
Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City, DF, Mexico
Facultad de Ciencias, UNAM, Mexico City, Mexico
Leyva-Garcia, Edgar:
Ctr Med Nacl Siglo XXI, Inst Mexicano Seguro Social, Unidad Invest Enfermedades Metabol, Mexico City, DF, Mexico
Unidad de Investigación en Enfermedades Metabólicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Garrido-Magana, Eulalia:
Ctr Med Nacl Siglo XXI, Inst Mexicano Seguro Social, UMAE Hosp Pediat, Mexico City, DF, Mexico
UMAE Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico city, Mexico
Rojas, Gerardo:
Ctr Med Nacl Siglo XXI, Inst Mexicano Seguro Social, UMAE Hosp Pediat, Mexico City, DF, Mexico
UMAE Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico city, Mexico
Felipe Jimenez-Garcia, Luis:
Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City, DF, Mexico
Revilla-Monsalve, Cristina:
Ctr Med Nacl Siglo XXI, Inst Mexicano Seguro Social, Unidad Invest Enfermedades Metabol, Mexico City, DF, Mexico
Unidad de Investigación en Enfermedades Metabólicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Altamirano, Perla:
Ctr Med Nacl Siglo XXI, Inst Mexicano Seguro Social, Unidad Invest Enfermedades Metabol, Mexico City, DF, Mexico
Unidad de Investigación en Enfermedades Metabólicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Calzada-Leon, Raul:
Inst Nacl Pediat, Mexico City, DF, Mexico
Instituto Nacional de Pediatría, México City, Mexico
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