Survival and Goat Milk Acidifying Activity of Lactobacillus rhamnosus GG Encapsulated with Agave Fructans in a Buttermilk Protein Matrix
Por:
Alvarado-Reveles O., Fernández-Michel S., Jiménez-Flores R., Cueto-Wong C., Vázquez-Moreno L., Montfort G.R.-C.
Publicada:
1 ene 2019
Resumen:
Lactobacillus rhamnosus GG (L. rhamnosus GG) cells were encapsulated in buttermilk proteins by spray drying, alone (E), or with Agave tequilana fructans (CEF). Buttermilk proteins acted as a thermo-protector for the probiotic cells undergoing the spray-dried process. The addition of Agave fructans in CEF microcapsules significantly enhanced storage stability and survival to in vitro simulated gastrointestinal conditions, compared to E capsules. After 14 days storage at - 20 °C, the number of living cells in CEF microcapsules was in the order of 7.7 log CFU • mL-1 and the survivability in simulated gastrointestinal environment was 73.23%. Spray-dried microparticles were cultured in goat milk to study biomass production. Agave fructans offered a favorable microenvironment and better growth substrate. The population of CEF viable cells reached 1.08 ± 0.02 × 1010 CFU • mL-1 after 18 h of fermentation. In contrast, the population of E viable cells were 3.0 ± 0.01 × 109 CFU • mL-1. The generation time of CEF, L. rhamnosus GG was 15% faster than E, L. rhamnosus GG. Encapsulation with buttermilk proteins in the presence of Agave fructans by spray drying could be suitable for preservation of probiotic powders and may be for a more effective application of probiotics in goat dairy products. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
Filiaciones:
Alvarado-Reveles O.:
Facultad de Ciencias Biológicas, Universidad Autónoma de Coahuila, Blvd. Torreón-Matamoros Km 7, Ejido el Águila, Ciudad Universitaria, Torreón, Coahuila 27000, Mexico
Fernández-Michel S.:
Facultad de Ciencias Biológicas, Universidad Autónoma de Coahuila, Blvd. Torreón-Matamoros Km 7, Ejido el Águila, Ciudad Universitaria, Torreón, Coahuila 27000, Mexico
Jiménez-Flores R.:
Dairy Products Technology Center, California Polytechnic State University, San Luis Obispo, CA 93407, United States
Cueto-Wong C.:
Facultad de Ciencias Biológicas, Universidad Autónoma de Coahuila, Blvd. Torreón-Matamoros Km 7, Ejido el Águila, Ciudad Universitaria, Torreón, Coahuila 27000, Mexico
Vázquez-Moreno L.:
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico
Montfort G.R.-C.:
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico
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