Immune response to chronic Toxocara canis infection in a mice model
Por:
Ruiz-Manzano R.A., Hernandez-Cervantes, Rosalia, Del Río-Araiza V.H., Palacios-Arreola M.I., Nava-Castro K.E., Morales-Montor, Jorge
Publicada:
1 dic 2019
Ahead of Print:
1 oct 2019
Resumen:
Aims The zoonotic nematode Toxocara canis causes larva migrans syndrome
that induces an immune response characterized by the production of
antibodies and eosinophilia. A Th2 polarization has been associated with
the infection, but there are still details of the cellular and humoral
immune response that need to be described. Thus, the aim of this study
was to describe the systemic host immune response to T canis chronic
infection in a mouse model. Methods and Results BALB/c mice were
inoculated once with 500 T canis embryonated eggs, per os. After 49
days, the amounts of larval found in brain and muscle tissues were
statistically two and four times higher, respectively, than the amounts
found in lung, liver, kidney or heart tissues. Splenic proportions of
F4/80(+) cells, as well as B, cytotoxic T and CD4(+)Foxp3(+)
lymphocytes, were statistically higher (P <= .05, P <= .01, P <= .001
and P <= .001, respectively) as compared with control mice. In lymph
nodes, some of these proportions changed, with the exception of F4/80(+)
cells. IgG1 levels in infected mice sera were increased. IL-4, IL-10 and
VEGF levels were statistically higher in spleen (P <= .05, all) and sera
(P <= .01, P <= .05 and P <= .05, respectively) in the infected mice.
Also, in infected animals, IL-5 serum levels were increased (P <= .01).
Conclusion These results suggest that T canis chronic infection in
BALB/c mice results in a type 2 response with an incipient regulatory
response.
Filiaciones:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Departamento de Parasitologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico, Ciudad de Mexico, Mexico
Departamento de Genotoxicología, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Circuito Mario de la Cueva S-N,AP 70228, Ciudad De Mexico 04510, Mexico
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Ciudad De Mexico, Mexico
Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Dept Genotoxicol, Ciudad De Mexico, Mexico
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