Marine archaeon methanosarcina acetivorans enhances polyphosphate metabolism under persistent cadmium stress
Por:
Jasso-Chavez, Ricardo, Lira-Silva, Elizabeth, Gonzalez-Sanchez, Kasia, Larios-Serrato, Violeta, Mendoza-Monzoy D.L., Perez-Villatoro, Fernando, Morett, Enrique, Vega-Segura, Alicia, Torres-Márquez M.E., Zepeda-Rodriguez, Armando, Moreno-Sanchez, Rafael
Publicada:
1 ene 2019
Resumen:
Phosphate metabolism was studied to determine whether polyphosphate (polyP) pools play a role in the enhanced resistance against Cd2+ and metal-removal capacity of Cd2+-preadapted (CdPA) Methanosarcina acetivorans. Polyphosphate kinase (PPK), exopolyphosphatase (PPX) and phosphate transporter transcript levels and their activities increased in CdPA cells compared to control (Cnt) cells. K+ inhibited recombinant Ma-PPK and activated Ma-PPX, whereas divalent cations activated both enzymes. Metal-binding polyP and thiol-containing molecule contents, Cd2+-removal, and biofilm synthesis were significantly higher in CdPA cells >Cnt cells plus a single addition of Cd2+ >Cnt cells. Also, CdPA cells showed a higher number of cadmium, sulfur, and phosphorus enriched-acidocalcisomes than control cells. Biochemical and physiological phenotype exhibited by CdPA cells returned to that of Cnt cells when cultured without Cd2+ . Furthermore, no differences in the sequenced genomes upstream and downstream of the genes involved in Cd2+ resistance were found between CdPA and Cnt cells, suggesting phenotype loss rather than genome mutations induced by chronic Cd2+-exposure. Instead, a metabolic adaptation induced by Cd2+ stress was apparent. The dynamic ability of M. acetivorans to change its metabolism, depending on the environmental conditions, may be advantageous to remove cadmium in nature and biodigesters. © 2019 Jasso-Chávez, Lira-Silva, González-Sánchez, Larios-Serrato, Mendoza-Monzoy, Pérez-Villatoro, Morett, Vega-Segura, Torres-Márquez, Zepeda-Rodríguez and Moreno-Sánchez.
Filiaciones:
Departamento de Bioquímica, Instituto Nacional de Cardiología, Mexico City, Mexico
Departamento de Farmacología, Instituto Nacional de Cardiología, Mexico City, Mexico
Winter Genomics, Mexico City, Mexico
Instituto Nacional de Medicina Genómica, Mexico City, Mexico
Instituto de Biotecnología, UNAM, Cuernavaca, Mexico
Facultad de Medicina, UNAM, Mexico City, Mexico
Inst Nacl Cardiol, Dept Bioquim, Mexico City, DF, Mexico
Inst Nacl Cardiol, Dept Farmacol, Mexico City, DF, Mexico
Winter Genom, Mexico City, DF, Mexico
Inst Nacl Med Genom, Mexico City, DF, Mexico
UNAM, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Mexico City, DF, Mexico
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