Comparative and functional genomics of the protozoan parasite Babesia divergens highlighting the invasion and egress processes
Por:
Gonzalez, Luis Miguel, Estrada, Karel, Grande, Ricardo, Jimenez-Jacinto, Veronica, Vega-Alvarado, Leticia, Sevilla, Elena, Barrera J., Cuesta, Isabel, Zaballos, Angel, Bautista, Jose Manuel, Lobo, Cheryl A., Sanchez-Flores, Alejandro, Montero, Estrella
Publicada:
1 ene 2019
Resumen:
Babesiosis is considered an emerging disease because its incidence has significantly increased in the last 30 years, providing evidence of the expanding range of this rare but potentially life-threatening zoonotic disease. Babesia divergens is a causative agent of babesiosis in humans and cattle in Europe. The recently sequenced genome of B. divergens revealed over 3,741 protein coding-genes and the 10.7-Mb high-quality draft become the first reference tool to study the genome structure of B. divergens. Now, by exploiting this sequence data and using new computational tools and assembly strategies, we have significantly improved the quality of the B. divergens genome. The new assembly shows better continuity and has a higher correspondence to B. bovis chromosomes. Moreover, we present a differential expression analysis using RNA sequencing of the two different stages of the asexual lifecycle of B. divergens: the free merozoite capable of invading erythrocytes and the intraerythrocytic parasite stage that remains within the erythrocyte until egress. Comparison of mRNA levels of both stages identified 1,441 differentially expressed genes. From these, around half were upregulated and the other half downregulated in the intraerythrocytic stage. Orthogonal validation by real-time quantitative reverse transcription PCR confirmed the differential expression. A moderately increased expression level of genes, putatively involved in the invasion and egress processes, were revealed in the intraerythrocytic stage compared with the free merozoite. On the basis of these results and in the absence of molecular models of invasion and egress for B. divergens, we have proposed the identified genes as putative molecular players in the invasion and egress processes. Our results contribute to an understanding of key parasitic strategies and pathogenesis and could be a valuable genomic resource to exploit for the design of diagnostic methods, drugs and vaccines to improve the control of babesiosis.
Filiaciones:
Gonzalez, Luis Miguel:
Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Madrid, Spain
ISCIII, Lab Referencia & Invest Parasitol, Ctr Nacl Microbiol, Madrid, Spain
Estrada, Karel:
Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Cuernavaca, Mexico
Inst Biotecnol, Unidad Univ Secuenciac Masiva & Bioinformat, Cuernavaca, Morelos, Mexico
Grande, Ricardo:
Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Cuernavaca, Mexico
Inst Biotecnol, Unidad Univ Secuenciac Masiva & Bioinformat, Cuernavaca, Morelos, Mexico
Jimenez-Jacinto, Veronica:
Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Cuernavaca, Mexico
Inst Biotecnol, Unidad Univ Secuenciac Masiva & Bioinformat, Cuernavaca, Morelos, Mexico
Vega-Alvarado, Leticia:
Instituto de Ciencias Aplicadas y Tecnología, UNAM, Ciudad de México, Mexico
Univ Nacl Autonoma Mexico, Inst Ciencias Aplicadas & Tecnol, Mexico City, DF, Mexico
Sevilla, Elena:
Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Madrid, Spain
ISCIII, Lab Referencia & Invest Parasitol, Ctr Nacl Microbiol, Madrid, Spain
Barrera J.:
Unidad de Bioinformática, Área de Unidades Centrales Científico-Técnicas, ISCIII, Majadahonda, Madrid, Spain
Cuesta, Isabel:
Unidad de Bioinformática, Área de Unidades Centrales Científico-Técnicas, ISCIII, Majadahonda, Madrid, Spain
ISCIII, Unidad Bioinformat, Area Unidades Cent Cient Tecn, Madrid, Spain
Zaballos, Angel:
Unidad de Genómica, Área de Unidades Centrales Científico-Técnicas, ISCIII, Majadahonda, Madrid, Spain
ISCIII, Unidad Genom, Area Unidades Cent Cient Tecn, Madrid, Spain
Bautista, Jose Manuel:
Department of Biochemistry and Molecular Biology & Research Institute Hospital 12 de Octubre, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain
Univ Complutense Madrid, Dept Biochem & Mol Biol, Hosp 12 Octubre, Fac Vet, Madrid, Spain
Univ Complutense Madrid, Res Inst, Hosp 12 Octubre, Fac Vet, Madrid, Spain
Lobo, Cheryl A.:
Blood Borne Parasites, New York Blood CenterNY, United States
New York Blood Ctr, Blood Borne Parasites, LFKRI, New York, NY 10021 USA
Sanchez-Flores, Alejandro:
Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Cuernavaca, Mexico
Inst Biotecnol, Unidad Univ Secuenciac Masiva & Bioinformat, Cuernavaca, Morelos, Mexico
Montero, Estrella:
Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Madrid, Spain
ISCIII, Lab Referencia & Invest Parasitol, Ctr Nacl Microbiol, Madrid, Spain
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