Physicochemical and Tissue Response of PLA Nanofiber Scaffolds Sterilized by Different Techniques
Por:
Mendieta-Barranon, Isarai, Chanes-Cuevas, Osmar A., Alvarez-Perez, Marco A., Gonzalez-Alva, Patricia, Medina, Luis A., Aguilar-Franco, Manuel, Serrano-Bello, Janeth
Publicada:
1 sep 2019
Resumen:
In recent years, tissue engineering has evolved considerably, due to the
problems in the biomedical area concerning tissue regeneration
therapies. Currently, work has been focused on the synthesis and
physicochemical characterization of poly lactic acid scaffolds, a
synthetic polyester that has been extensively study for its excellent
biocompatibility and biodegradability. Moreover, sterilization
strategies of scaffold are a crucial step for its application in tissue
regeneration, however, the sterilization process have to maintain the
structural and biochemical properties of the scaffold. Therefore, it is
very important to carry out studies on the sterilization methods of the
sample's material, since translational medicine is intended for in vivo
applications. The aim of the present study was designed to analyze the
effects of different sterilization techniques, i.e. ethylene oxide
(ETO), gamma radiation (GR) and hydrogen peroxide-based plasma (H2O2) in
biodegradable PLA scaffolds, and to determine the best sterilization
technique to render a sterile product with minimal degradation and
deformation, and good tissue response. Analysis of surface morphology
showed that ETO and GR modified the PLA scaffolds without any change in
its chemical composition. Moreover, the histological response showed
that the scaffolds are biocompatible and those sterilized by GR showed a
more severe inflammatory response, accompanied with the presence of
giant foreign body cells. In conclusion, the results show that among
sterilization techniques used in the preset study, the best results were
observed with H2O2 sterilization, since it did not significantly modify
the surface structure of the PLA fibers and their in vivo response did
not cause an unfavorable tissue reaction.
Filiaciones:
Mendieta-Barranon, Isarai:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Bioingn Tejidos DEPeI, Circuito Exterior S-N,Cd Univ, Coyoacan 04510, Cdmx, Mexico
Chanes-Cuevas, Osmar A.:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Bioingn Tejidos DEPeI, Circuito Exterior S-N,Cd Univ, Coyoacan 04510, Cdmx, Mexico
Alvarez-Perez, Marco A.:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Bioingn Tejidos DEPeI, Circuito Exterior S-N,Cd Univ, Coyoacan 04510, Cdmx, Mexico
Gonzalez-Alva, Patricia:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Bioingn Tejidos DEPeI, Circuito Exterior S-N,Cd Univ, Coyoacan 04510, Cdmx, Mexico
Medina, Luis A.:
Univ Nacl Autonoma Mexico, Inst Fis, Circuito Exterior S-N,Cd Univ, Coyoacan 04510, Cdmx, Mexico
UNAM, Inst Nacl Cancerol, Unidad Invest Biomed Canc INCan, Cdmx 14080, Mexico
Aguilar-Franco, Manuel:
Univ Nacl Autonoma Mexico, Inst Fis, Circuito Exterior S-N,Cd Univ, Coyoacan 04510, Cdmx, Mexico
Serrano-Bello, Janeth:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Bioingn Tejidos DEPeI, Circuito Exterior S-N,Cd Univ, Coyoacan 04510, Cdmx, Mexico
All Open Access; Bronze
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