Monitoring of the enzymatic activity of intracellular lipases of Ustilago maydis expressed during the growth under nitrogen limitation and its correlation in lipolytic reactions
Por:
Araiza-Villanueva, M. G., Olicon-Hernandez, D. R., Pardo, J. P., Vazquez-Meza, H., Guerra-Sanchez, G.
Publicada:
1 oct 2019
Resumen:
Under nitrogen starvation, Ustilago maydis forms lipid droplets (LDs).
Although the dynamics of these organelles are known in the literature,
the identity of the lipases implicated in their degradation is unknown.
We determined lipase activity and identified the intracellular lipases
expressed during growth under nitrogen starvation and YPD media by
zymograms. The results showed that cytosolic extracts exhibited higher
lipase activity when cells were grown in YPD. Under nitrogen starvation,
lipase activity was not detected after 24 h of culture, resulting in
lipid accumulation in LDs. This suggests that these lipases could be
implicated in LD degradation. In the zymogram, two bands, one of 25 and
the other of 37 kDa, presented lipase activity. The YPD extracts showed
lipase activity in olive and almond oils, which contain triacylglycerols
with mono and polyunsaturated fatty acids. This is the first report
about U. maydis cytosolic lipases involved in LD degradation.
Filiaciones:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Carpio y Plan Ayala S-N, Ciudad De Mexico 11340, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Circuito Interior S-N,Ciudad Univ, Cd De Mexico 04510, Mexico
|