Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in Mexico
Por:
Carreto-Binaghi, Laura E., Morales-Villarreal, Fernando R., Garcia-de la Torre, Guadalupe, Vite-Garin, Tania, Ramirez J.-A., Aliouat E.-M., Martínez-Orozco J.-A., Taylor M.-L.
Publicada:
1 ene 2019
Resumen:
Background: Histoplasma capsulatum and Pneumocystis jirovecii are respiratory fungal pathogens that principally cause pulmonary disease. Coinfection with both pathogens is scarcely reported. This study detected this coinfection using specific molecular methods for each fungus in the bronchoalveolar lavage (BAL) of patients from a tertiary care hospital. Materials and methods: BAL samples from 289 hospitalized patients were screened by PCR with specific markers for H. capsulatum (Hcp100) and P. jirovecii (mtLSUrRNA and mtSSUrRNA). The presence of these pathogens was confirmed by the generated sequences for each marker. The clinical and laboratory data for the patients were analyzed using statistical software. Results: The PCR findings separated three groups of patients, where the first was represented by 60 (20.8%) histoplasmosis patients, the second by 45 (15.6%) patients with pneumocystosis, and the last group by 12 (4.2%) patients with coinfection. High similarity among the generated sequences of each species was demonstrated by BLASTn and neighbor-joining algorithms. The estimated prevalence of H. capsulatum and P. jirovecii coinfection was higher in HIV patients. © 2019 The Authors
Filiaciones:
Laboratorio de Inmunología de Hongos, Unidad de Micología, Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), CDMX04510, Mexico
Departamento de Investigación en Microbiología, Instituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas” (INER), CDMX14080, Mexico
Departamento de Microbiología Clínica, INER, CDMX14080, Mexico
Departamento de Salud Pública, Facultad de Medicina, UNAM, CDMX04510, Mexico
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Centre d'Infection et d'Immunité de Lille, Lille, France
Univ Nacl Autonoma Mexico, Fac Med, Lab Inmunol Hongos, Unidad Micol,Dept Microbiol Parasitol, Mexico City 04510, DF, Mexico
Inst Nacl Enfermedades Resp Ismael Cosio Villegas, Dept Invest Microbiol, Mexico City 14080, DF, Mexico
INER, Dept Microbiol Clin, Mexico City 14080, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Salud Publ, Mexico City 04510, DF, Mexico
Univ Lille, Inst Pasteur Lille, CHU Lille, INSERM,CNRS,CIIL,UMR 8204,U1019, Lille, France
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