Molecular Features and mRNA Expression of the Receptor for Activated C Kinase 1 from Symbiodinium microadriaticum ssp. microadriaticum During Growth and the Light/Dark cycle
Por:
Islas-Flores, Tania, Perez-Cervantes, Esmeralda, Nava-Galeana, Jessica, Loredo-Guillen, Montserrat, Guillen, Gabriel, Villanueva, Marco A.
Publicada:
1 mar 2019
Categoría:
Microbiology
Resumen:
Two genes of the RACK1 homolog from the photosynthetic dinoflagellate
Symbiodinium microadriaticum ssp. microadriaticum (SmicRACK1), termed
SmicRACK1A and SmicRACK1B, were found tandemly arrayed and displayed a
single synonymous substitution (T/C) encoding threonine. They included
two exons of 942 bp each, encoding 313 amino acids with seven WD-40
repeats and two PKC-binding motifs. The protein theoretical mass and pI
were 34,200 Da and 5.9, respectively. SmicRACK1 showed maximum
identities with RACK1 homologs at the amino acid and nucleotide level,
respectively, of 92 and 84% with S. minutum, and phylogenetic analysis
revealed clustered related RACK1 sequences from the marine
dinoflagellates S. minutum, Heterocapsa triquetra, Karenia brevis, and
Alexandrium tamarense. Interestingly, light-dependent regulatory
elements were found both within the 282 bp SmicRACK1A promotor sequence,
and within an intergenic sequence of 359 nucleotides that separated both
genes, which strongly suggest light-related functions. This was further
supported by mRNA accumulation analysis, which fluctuated along the
light and dark phases of the growth cycle showing maximum specific peaks
under either condition. Finally, qRT-PCR analysis revealed differential
SmicRACK1 mRNA accumulation with maxima at 6 and 20 d of culture. Our
SmicRACK1 characterization suggests roles in active growth and
proliferation, as well as light/dark cycle regulation in S.
microadriaticum.
Filiaciones:
UNAM, Inst Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifales, Prolongac Ave Ninos Heroes S-N, Puerto Morelos 77580, Quintana Roo, Mexico
Univ Nacl Autonoma Mexico, Posgrad Ciencias Mar & Limnol, Circuito Exterior S-N,Ciudad Univ, Mexico City 04510, DF, Mexico
Int Hosp Morelos, Grp QUAE, S RL, Lab Diagnost Mol, Calle Luz 44, Cuernavaca 62450, Morelos, Mexico
UNAM, Inst Biotecnol, Dept Biol Mol Plantas, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico
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