Cadmium exposure induces interleukin-6 production via ROS-dependent activation of the ERK1/2 but independent of JNK signaling pathway in human placental JEG-3 trophoblast cells
Por:
Paniagua, Lucero, Diaz-Cueto, Laura, Huerta-Reyes, Maira, Arechavaleta-Velasco, Fabian
Publicada:
1 ene 2019
Categoría:
Toxicology
Resumen:
Maternal exposure to cadmium (Cd) has been associated with preeclampsia (PE), which is a multisystemic disorder characterized by endothelial dysfunction. Elevated interleukin (IL)-6 expression is linked to PE and has been suggested to contribute to maternal endothelial dysfunction. Cd induces IL-6 production in various cell types through different signaling pathways. Thus, this study was designed to investigate the effect of Cd on IL-6 production and the underlying mechanisms in a trophoblast-derived cell line. Cultured JEG-3 trophoblast cells were exposed to non-toxic concentrations of CdCl2 in the presence or absence of various MAPK inhibitors or N-Acetyl-L-cysteine (NAC). IL-6 was measured by ELISA. Phosphorylation of ERK1/2, JNK, and c-Jun was assessed by Western blotting. Cd exposure induced IL-6 production and increased ERK1/2, JNK, and c-Jun phosphorylation. NAC and the inhibition of ERK1/2 significantly reduced Cd-induced IL-6 production. These data indicate that Cd induces IL-6 production in trophoblast cells through a ROS-dependent activation of ERK1/2. © 2019 Elsevier Inc.
Filiaciones:
Paniagua, Lucero:
Unidad de Investigacion Medica en Medicina Reproductiva, IMSS, UMAE Hospital de Gineco Obstetricia No. 4 “Dr. Luis Castelazo Ayala”, CDMX, Mexico
Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, CDMX, Mexico
UMAE Hosp Gineco Obstet, Unidad Invest Med Med Reprod, IMSS, 4 Dr Luis Castelazo Ayala, Cdmx, Mexico
Univ Nacl Autonoma Mexico, Ciencias Biol, Cdmx, Mexico
Diaz-Cueto, Laura:
Unidad de Investigacion Medica en Medicina Reproductiva, IMSS, UMAE Hospital de Gineco Obstetricia No. 4 “Dr. Luis Castelazo Ayala”, CDMX, Mexico
UMAE Hosp Gineco Obstet, Unidad Invest Med Med Reprod, IMSS, 4 Dr Luis Castelazo Ayala, Cdmx, Mexico
Huerta-Reyes, Maira:
Unidad de Investigacion Medica en Enfermedades Nefrologicas, Hospital de Especialidades “Dr. Bernardo Sepúlveda Gutiérrez”, Centro Medico Nacional Siglo XXI, IMSS, CDMX, Mexico
Hosp Especialidades Dr Bernardo Septilveda Gutier, Ctr Med Nacl Siglo XXI, IMSS, Unidad Invest Med Enfermedades Nefrol, Cdmx, Mexico
Arechavaleta-Velasco, Fabian:
Unidad de Investigacion Medica en Medicina Reproductiva, IMSS, UMAE Hospital de Gineco Obstetricia No. 4 “Dr. Luis Castelazo Ayala”, CDMX, Mexico
UMAE Hosp Gineco Obstet, Unidad Invest Med Med Reprod, IMSS, 4 Dr Luis Castelazo Ayala, Cdmx, Mexico
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