Description of a new species and understanding the genetic diversity of Saccocoelioides Szidat, 1954 (Haploporidae) in Middle America using mitochondrial and nuclear DNA sequences
Por:
Andrade-Gomez, Leopoldo, Lucia Sereno-Uribe, Ana, Garcia-Varela, Martin
Publicada:
1 ago 2019
Resumen:
Members of the genus Saccocoelioides Szidat, 1954, include endoparasites
from freshwater and brackish fishes from the Americas. Adult specimens
were collected from the intestines of Poecilia catemaconis Miller, 1975,
a poeciliid fish endemic to Catemaco Lake, and the white mullet Mugil
curema Valenciennes, 1836, from Alvarado Lagoon, Veracruz, Mexico. The
specimens were sequenced for three molecular markers, internal
transcribed spacer 2 (ITS2) and domains D1-D3 from the large subunit
(LSU) of nuclear ribosomal DNA and cytochrome c oxidase subunit 1 (cox
1) from mitochondrial DNA. The newly sequenced specimens were aligned
with other sequences downloaded from GenBank. Maximum likelihood and
Bayesian inference analyses were inferred with three data sets (a
combination of nuclear DNA ITS2 + LSU, cox 1 alone and the concatenated
cox 1 + ITS2 + LSU). The phylogenetic analyses inferred with the
combined data set of the two nuclear molecular markers (ITS2 + LSU)
revealed that Saccocoelioides is monophyletic and formed 11 independent
lineages representing 11 valid species previously recognized plus the
new lineage that is herein described as a new species named
Saccocoelioides macrospinosus n. sp., however, the new species was
placed in a basal polytomy in the tree. Therefore, the addition of a
mitochondrial gene with a fast rate of substitution was fundamental to
clarify the phylogenetic relationships of the new species. The genetic
divergences estimated with the cox 1 gene were high, ranging from 8.3 to
15.5% among Saccocoelioides macrospinosus n. sp. and sister taxa. The
new species has a slightly elongated body measuring 440-850 mu m long
and was classified in the diminutive morphotype. In addition, seven
adult specimens recovered from the intestines of the banded tetra fish
Astyanax aeneus Gunther, 1860 from Nicaragua and Costa Rica formed a
monophyletic Glade with other specimens identified previously as
Saccocoelioides tkachi, expanding its distribution range in other areas
of Middle America.
Filiaciones:
Andrade-Gomez, Leopoldo:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Posgrad Ciencias Biol, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Avenida Universidad 3000, Distrito Federal, C. P. 04510, Mexico
Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Avenida Universidad 3000, Distrito Federal, C. P. 04510, Mexico
Lucia Sereno-Uribe, Ana:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Garcia-Varela, Martin:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Avenida Universidad 3000, Distrito Federal, C. P. 04510, Mexico
|