Genotyping Based on the LTR Region of Small Ruminant Lentiviruses from Naturally Infected Sheep and Goats from Mexico
Por:
Mendiola, Wolfang P. S., Tortora, Jorge L., Martinez, Humberto A., Garcia, Maria M., Cuevas-Romero, Sandra, Cerriteno, Jose L., Ramirez, Hugo
Publicada:
1 ene 2019
Resumen:
Small ruminant lentiviruses (SRLVs) belong to the genus Lentivirus in
the Retroviridae family. There are five genotypes (A, B, C, D, and E),
where genotypes A and B have a global distribution and genotypes C, D,
and E are limited to Europe. The presence of SRLV has been confirmed in
Mexico, with genotype B detected in the central region of the country.
We examined the presence of SRLVs and genotype prevalence in 1014 sheep
and 1383 goats from 12 Mexican states. Using a commercial competitive
ELISA (cELISA) test, we detected SRLV antibodies in 107 sheep (10.55%)
and 466 goats (33.69%). We used an endpoint PCR to amplify the LTR
region on seropositive animals. A total of 50 sheep and 75 goats tested
positive via PCR. Positive amplicons from 11 sheep and 17 goats from ten
Mexican States were cloned and sequenced. With the LTR sequence data
obtained in this study, a phylogenetic analysis was performed; we also
constructed a phylogenetic tree using the obtained sequences and
GenBank's available sequences. All studied sequences were associated
with genotype B, specifically with the FESC-752 isolate previously
identified in Mexico. Highly conserved transcription factor binding
sites were observed in analyzed alignments, such as AML (vis), AP-4, and
TATA box. However, we identified nucleotide differences at site AP-1
that suggest function loss. Our study found that ovine and caprine
genotype B SRLVs are widely distributed in Mexico; a highly conserved
LTR region among the sequences evaluated in this study was also found.
Filiaciones:
Mendiola, Wolfang P. S.:
Univ Nacl Autonoma Mexico, Virol Genet & Mol Biol Lab, Fac Higher Educ, Vet Med, Campus 4, Cuautitlan 54714, Mex, Mexico
Tortora, Jorge L.:
Univ Nacl Autonoma Mexico, Virol Genet & Mol Biol Lab, Fac Higher Educ, Vet Med, Campus 4, Cuautitlan 54714, Mex, Mexico
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km 2.5 Carretera Cuautitlan-Teoloyucan, San Sebastian Xhala Cuautitlan Izcalli, MEX, C.P. 54714, Mexico
Martinez, Humberto A.:
Univ Nacl Autonoma Mexico, Virol Genet & Mol Biol Lab, Fac Higher Educ, Vet Med, Campus 4, Cuautitlan 54714, Mex, Mexico
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km 2.5 Carretera Cuautitlan-Teoloyucan, San Sebastian Xhala Cuautitlan Izcalli, MEX, C.P. 54714, Mexico
Garcia, Maria M.:
Mexican Inst Social Secur, Natl Med Ctr XXI Century, Pediat Hosp, Lab Immunovirol,Med Res Immunol Unit, Mexico City, DF, Mexico
Laboratory of Immunovirology, Medical Research in Immunology Unit, Pediatric Hospital, National Medical Center XXI Century, Mexican Institute of Social Security, Mexico
Cuevas-Romero, Sandra:
Natl Res Inst Forestry & Agr, INIFAP, Natl Res Ctr Anim Microbiol Disciplines, Mexico City 05110, DF, Mexico
National Research Center of Animal Microbiology Disciplines, National Research Institute of Forestry and Agriculture, INIFAP, Mexico City, C.P. 05110, Mexico
Cerriteno, Jose L.:
Natl Res Inst Forestry & Agr, INIFAP, Natl Res Ctr Anim Microbiol Disciplines, Mexico City 05110, DF, Mexico
Ramirez, Hugo:
Univ Nacl Autonoma Mexico, Virol Genet & Mol Biol Lab, Fac Higher Educ, Vet Med, Campus 4, Cuautitlan 54714, Mex, Mexico
Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education, Cuautitlan, Veterinary Medicine, Campus 4, National Autonomous University of Mexico, Km 2.5 Carretera Cuautitlan-Teoloyucan, San Sebastian Xhala Cuautitlan Izcalli, MEX, C.P. 54714, Mexico
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