Increased expression of FAK isoforms as potential cancer biomarkers in ovarian cancer
Por:
Nolasco-Quiroga M., Rosas-Díaz M., Moreno J., Godínez-Aguilar R., López-Ibarra M.J., Piña-Sánchez P., Alvarado-Cabrero I., Vázquez-Gómez G., Rocha-Zavaleta L., Arenas-Aranda D., Salamanca - Gómez F.
Publicada:
1 ene 2019
Resumen:
Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that is expressed in most human cell types (example: Epithelial cells, fibroblasts and endothelial), it serves a key role in the control of cell survival, proliferation and motility. The abnormal expression of FAK has been associated with poor prognosis in cancer, including ovarian cancer. However, although FAK isoforms with specific molecular and functional properties have been characterized, there are a limited number of published studies that examine FAK isoforms in ovarian cancer. The aim of the present study was to analyze the expression level of FAK and its isoforms in ovarian cancer. The expression of FAK kinase and focal adhesion targeting (FAT) domains was determined with immunohistochemistry in healthy ovary, and serous and mucinous cystadenoma, borderline tumor and carcinoma samples. Additionally, the expression of FAK and its isoforms were investigated in three ovarian cancer-derived cell lines with western blotting and reverse transcription-semi-quantitative polymerase chain reaction. An increased expression of FAK kinase domain was determined in serous tumor samples and was associated with advancement of the lesion. FAK kinase domain expression was moderate-to-low in mucinous tumor samples. The expression of the FAK FAT domain in tumor samples was reduced, compared with healthy ovary samples; however, the FAT domain was localized to the cellular nucleus. Expression of alternative transcripts FAK0, FAK28,6 and FAK28 was determined in all three cell lines investigated. In conclusion, FAK kinase and FAT domains are differentially expressed among ovarian tumor types. These results indicated the presence of at least two isoforms of FAK (FAK and the putative FAK-related non-kinase) in tumor tissue, which is supported by the cells producing at least three FAK alternative transcripts. These results may support the use of FAK and its isoforms as biomarkers for ovarian cancer. © 2019, Spandidos Publications. All rights reserved.
Filiaciones:
Laboratory of Molecular Genetics, Medical Research Unit, Mexican Social Security Institute, National Medical Center Century XXI, Hospital of Pediatrics, Mexico City, 06720, Mexico
, Mexico
Multidisciplinary Academic Unit Reynosa-Aztlan Reynosa, Autonomous University of Tamaulipas, Tamaulipas, 88740, Mexico
Direction of Research, Hospital Juárez de México, Mexico City, 07760, Mexico
Medical Research Unit of Oncologic Diseases, Mexican Social Security Institute, National Medical Center Century XXI, Oncology Hospital, Mexico City, 06720, Mexico
Department of Anatomy-Pathology, Mexican Social Security Institute, National Medical Center Century XXI, Oncology Hospital, Mexico City, 06720, Mexico
Departments of Genomic Medicine and Envornmental Toxicology, National University of Mexico
Departments of Molecular Biology and Biotechnology, National University of Mexico
Medical Research Coordination, Mexican Social Security Institute, National Medical Center Century XXI, Mexico City, 04510, Mexico
Natl Med Ctr Century XXI, Hosp Pediat, Mexican Social Secur Inst, Med Res Unit,Lab Mol Genet, Mexico City 06720, DF, Mexico
Autonomous Univ Tamaulipas, Multidisciplinary Acad Unit Reynosa Aztlan Reynos, Tamaulipas 88740, Mexico
Natl Med Ctr Century XXI, Oncol Hosp, Mexican Social Secur Inst, Med Res Unit Oncol Dis, Mexico City 06720, DF, Mexico
Natl Med Ctr Century XXI, Oncol Hosp, Mexican Social Secur Inst, Dept Anat Pathol, Mexico City 06720, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Gen Med & Envornmental Toxicol, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Mol Biol & Biotechnol, Mexico City, DF, Mexico
Natl Med Ctr Century XXI, Mexican Social Secur Inst, Med Res Coordinat, 330 Ave Cuauhtemoc, Mexico City 04510, DF, Mexico
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