Pirfenidone prevents acute kidney injury in the rat
Por:
Lima-Posada I., Fontana F., Pérez-Villalva R., Berman-Parks N., Bobadilla N.A.
Publicada:
8 may 2019
Categoría:
Nephrology
Resumen:
BackgroundPirfenidone is an orally active drug used for the treatment of
idiopathic pulmonary fibrosis to slow loss of lung function; it acts
mainly through an antifibrotic effect but also possesses antioxidant and
anti-inflammatory properties. We assessed the effect of prophylactic
administration of pirfenidone on acute kidney injury due to bilateral
renal ischemia.MethodsEighteen rats were included and divided in: 1)
sham-operated rats (S), 2) rats underwent bilateral renal ischemia for
20min (I/R), and 3) rats treated with pirfenidone 700mg/kg/day 24h
before surgery and subjected to bilateral renal ischemia for 20min
(I/R+PFN). All the rats were euthanized and studied 24h after renal
reperfusion.ResultsAs was expected, the I/R group exhibited a
significant reduction in creatinine clearance, urinary output and renal
blood flow, as well as extensive tubular injury. These alterations were
associated with a significant decrease in urinary excretion of nitrites
and nitrates (UNO2/NO3V). In the I/R+PFN group, recovery of renal
function and UNO2/NO3V was observed, together with lesser histological
signs of tubular injury compared to the I/R group.ConclusionsThis study
shows that prophylactic administration of pirfenidone prevented acute
kidney injury due to bilateral ischemia in the rat. Recovery of NO
production appears to be one of the mechanism of pirfenidone
renoprotective effect. Our findings suggest that pirfenidone is a
promising drug to reduce renal injury induced by I/R.
Filiaciones:
Lima-Posada I.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mol Physiol Unit, Vasco de Quiroga 15, Mexico City 14000, DF, Mexico
Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Nephrol, Mexico City, DF, Mexico
Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Vasco de Quiroga No. 15, Tlalpan Mexico City, 14000, Mexico
Department of Nephrology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Fontana F.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mol Physiol Unit, Vasco de Quiroga 15, Mexico City 14000, DF, Mexico
Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Nephrol, Mexico City, DF, Mexico
Univ Modena & Reggio Emilia, Sect Nephrol, Surg Med & Dent Dept Morphol Sci, Modena, Italy
Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Vasco de Quiroga No. 15, Tlalpan Mexico City, 14000, Mexico
Department of Nephrology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Surgical, Medical and Dental Department of Morphological Sciences, Section of Nephrology, University of Modena and Reggio Emilia, Modena, Italy
Pérez-Villalva R.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mol Physiol Unit, Vasco de Quiroga 15, Mexico City 14000, DF, Mexico
Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Nephrol, Mexico City, DF, Mexico
Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Vasco de Quiroga No. 15, Tlalpan Mexico City, 14000, Mexico
Department of Nephrology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Berman-Parks N.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mol Physiol Unit, Vasco de Quiroga 15, Mexico City 14000, DF, Mexico
Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Nephrol, Mexico City, DF, Mexico
Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Vasco de Quiroga No. 15, Tlalpan Mexico City, 14000, Mexico
Department of Nephrology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Bobadilla N.A.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mol Physiol Unit, Vasco de Quiroga 15, Mexico City 14000, DF, Mexico
Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Nephrol, Mexico City, DF, Mexico
Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Vasco de Quiroga No. 15, Tlalpan Mexico City, 14000, Mexico
Department of Nephrology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
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