Moderate protection is induced by a chimeric protein composed of leucine aminopeptidase and cathepsin L1 against Fasciola hepatica challenge in sheep
Por:
Ortega-Vargas S., Espitia C., Sahagún-Ruiz A., Parada C., Balderas-Loaeza A., Villa-Mancera A., Quiroz-Romero H.
Publicada:
1 ene 2019
Resumen:
Leucine aminopeptidase (FhLAP) and cathepsin L1 (FhCL1) of Fasciola hepatica play a critical role in parasite feeding, migration through host tissue, and immune evasion. These antigens have been tested for immune protection as single components with variable degrees of success. The chimeric-protein approach could improve protection levels against fasciolosis. Previously, we reported the design and construction of a chimeric protein composed of antigenic sequences of FhLAP and FhCL1 of F. hepatica. The goal of the present study was to express and evaluate the immune-protective capacity of this chimeric protein (rFhLAP-CL1) in sheep. Animals were randomly allocated into five groups with five animals in each group. Groups 1, 2 and 3 were immunized twice with 100 µg, 200 µg and 400 µg of rFhLAP-CL1 emulsified with Quil A adjuvant, whereas groups 4 and 5 were the adjuvant control and infection control groups, respectively. The animals were then challenged with 200 metacercariae two weeks after the rFhLAP-CL1 booster. The fluke burden was reduced by 25.5%, 30.7% (p < 0.05) and 46.5% (p < 0.01) in sheep immunized with 100 µg, 200 µg and 400 µg of chimeric protein, respectively, in comparison to the infection control group. There was a reduction of 22.7% (p < 0.05) and 24.4% (p < 0.01) in fecal egg count in groups 2 and 3, respectively, compared to the infection control group. Sheep immunized with chimeric protein produced F. hepatica excretion-secretion product-specific total IgG antibody, which were increased after challenge. Moreover, the levels of rFhLAP-CL1-specific IgG1 and IgG2 isotypes in immunized sheep increased rapidly two weeks after the first immunization and were significantly more elevated than those of the control groups, indicating a mixed Th1/Th2 response. This is a preliminary evaluation of the chimeric protein rFhLAP-CL1 as a possible immunogen against F. hepatica infection in sheep. © 2019 Elsevier Ltd
Filiaciones:
Ortega-Vargas S.:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación Coyoacán, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Av Univ 3000, Ciudad De Mexico 04510, Mexico
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación Coyoacán, Ciudad de México, C.P. 04510, Mexico
Espitia C.:
Departamento de inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de MéxicoDF C.P. 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Departamento de inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, DF C.P. 04510, Mexico
Sahagún-Ruiz A.:
Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación CoyoacánD.F C.P. 04510, Mexico
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Microbiol & Inmunol, Av Univ 3000, Mexico City 04510, DF, Mexico
Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación Coyoacán, México, D.F C.P. 04510, Mexico
Parada C.:
Departamento de inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de MéxicoDF C.P. 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Departamento de inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, DF C.P. 04510, Mexico
Balderas-Loaeza A.:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación Coyoacán, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Av Univ 3000, Ciudad De Mexico 04510, Mexico
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación Coyoacán, Ciudad de México, C.P. 04510, Mexico
Villa-Mancera A.:
Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, 4 Sur 304 Col. Centro, C.P. 75482, Tecamachalco, Puebla, Mexico
Benemerita Univ Autonoma Puebla, Fac Med Vet & Zootecnia, 4 Sur 304 Col Ctr, Puebla 75482, Mexico
Quiroz-Romero H.:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación Coyoacán, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Av Univ 3000, Ciudad De Mexico 04510, Mexico
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación Coyoacán, Ciudad de México, C.P. 04510, Mexico
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