Distribution of Virulence and Antimicrobial Resistance Genes in Phylogenetic Groups of Escherichia coli Strains Isolated from Mexican Patients with Urinary Infection
Por:
Carlos Bravata-Alcantara, Juan, Manuel Bello-Lopez, Juan, Alejandra Cortes-Ortiz, Iliana, Jose Mendez-Velazquez, Juan, Aviles-Soto, Brandon, Itzel Quintas-Granados, Laura, del Carmen Chavez-Ocana, Sonia, Rosel-Pech, Cecilia, Antonio Gonzalez-Barrios, Juan, Sierra Martinez, Monica
Publicada:
1 mar 2019
Resumen:
Background: The causative primary agent of urinary tract infections
(UTI) is uropathogenic Escherichia coli (UPEC); however, commensal
Escherichia coli (CEC) has been also implicated in the development of
UTI. Due to the recent emergence of virulent and resistant strains, it
is necessary to have evidence to demonstrate that UPEC and CEC are
isolated from patients with UTI and subsequently they have the genes to
be considered pathogenic.
Objectives: To determine the distribution of resistance and virulence
genes in UPEC and CEC strains isolated from the patients with UTI
(hospitalized and ambulatory).
Methods: One hundred seven E. coli strains were genotyped according to
Clermont protocol and were subjected to PCR assays in order to detect
resistance and virulence genes. In addition, the antimicrobial test in
solid media was performed to determinate the correlation
``phenolgenotype''.
Results: Genotyping analysis showed that group B2 (42.05%) was the most
predominant, strains followed by A (27.1%), D (24.29%), and finally,
B1(6.54%). beta-lactams, carbapenems, aminoglycosides, and nitrofurans
showed the best activity. The virulence gene frequencies were: fimH
(92/85.98%), iutA (68/63.55%), traT (66/61.68%), papC (36/33.64%),
and cnf1 (15/14.02%) while resistance genes were: bla(CIX-M)
(41/38.32%), bla(OXA) (49/45.79%), and bla(SHV) (2/1.87%). The
associations fimH/bla(CIX),fimH/bla(OXA), traT/bla(CIX), traT/bla(OXA),
iutA/bla(CIX), and iutA/bla(OXA) showed higher incidence while the
associations with cnf1 and papC genes were low.
Conclusions: Uropathogenic E. coli has the necessary genetic elements
(virulence and resistance) to be considered the main pathogen causing
UTI in the Mexican population. Additionally, to our knowledge, there are
no studies in our country demonstrating that the CEC isolated from the
patients with UTI contains genetic elements of virulence and resistance
that allow them to be potentially pathogenic.
Filiaciones:
Carlos Bravata-Alcantara, Juan:
Juarez de Mexico Hosp, Genet & Mol Diag Lab, Mexico City, DF, Mexico
Manuel Bello-Lopez, Juan:
Juarez de Mexico Hosp, Res Unit Microbiol & Toxicol, Mexico City, DF, Mexico
Alejandra Cortes-Ortiz, Iliana:
Juarez de Mexico Hosp, Bacteriol Lab, Mexico City, DF, Mexico
Jose Mendez-Velazquez, Juan:
Univ Nacl Autonoma Mexico, Zaragoza Sch Higher Studies, Mexico City, DF, Mexico
Aviles-Soto, Brandon:
Technol Univ Tecamac, Tecamac, Mexico
Itzel Quintas-Granados, Laura:
Mexiquense Bicentenario Univ, Higher Studies Unit Tultitlan, Mexico City, DF, Mexico
del Carmen Chavez-Ocana, Sonia:
Juarez de Mexico Hosp, Genet & Mol Diag Lab, Mexico City, DF, Mexico
Rosel-Pech, Cecilia:
Hosp Infectol Daniel Mendez Hernandez, la Raza Natl Med Ctr, Med Res Unit Immunol & Infect Dis, Mexico City, DF, Mexico
Antonio Gonzalez-Barrios, Juan:
ISSSTE, Reg Hosp, Lab Genom Med, Mexico City, DF, Mexico
Sierra Martinez, Monica:
Juarez de Mexico Hosp, Genet & Mol Diag Lab, Mexico City, DF, Mexico
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