Maternal manipulation during late gestation (GDs 17-20) enhances ethanol consumption and promotes changes and opioid mRNA expression in infant rats
Por:
Guttlein L., Macchione A.F., Hernández-Fonseca K., Haymal O.B., Molina J.C., Méndez Ubach M., Abate P.
Publicada:
1 ene 2019
Categoría:
Behavioral neuroscience
Resumen:
Fetal ethanol experience generates learning and memories capable to increase ethanol consummatory behaviors during infancy. Opioid system seems to be involved in mediating those alcohol-related behaviors. In this work, we proposed to study the impact of prenatal exposure to a moderate ethanol dose, upon ingestion of the drug and possible ethanol-induced molecular changes on opioid precursor peptides (POMC, Pro-enk and Pro-DYN) and receptors (MOR, DOR and KOR) mRNA expression, in hypothalamus. Pregnant rats received during gestational days (GDs) 17-20, a daily intragastric (i.g.) administration with 2g/kg ethanol or water. A third group of dams was left undisturbed during pregnancy (Unmanipulated group). Intake test was conducted at postnatal days (PDs) 14-15. Three groups of pups were performed: control (no intake test), water (vehicle) and 5% ethanol. At the end of intake test blood samples were taken to quantify blood ethanol concentrations (BECs) and hypothalamus sections were obtained to perform qRT-PRC assessment of opioid precursor peptides and receptors. The analysis of the consummatory responses (% of consumption) and pharmacokinetic profiles (BECs) suggested that maternal manipulation induced by i.g. intubations, during the last four days of gestation (whenever ethanol or water), are sufficient to induce infantile ethanol intake during infancy. Gene expression from the hypothalamus of unmanipulated group revealed that infantile ingestive experiences with ethanol can down-regulate expression of mRNA Pro-Dyn and up-regulate mRNA expression of MOR and KOR. Finally, MOR mRNA expression was attenuated by prenatal i.g. manipulation in pups exposed to 5% ethanol. © 2019 Elsevier B.V.
Filiaciones:
Guttlein L.:
Instituto de Investigación Médica M. y M. Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, 5016, Argentina
Macchione A.F.:
Instituto de Investigación Médica M. y M. Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, 5016, Argentina
Facultad de Psicología, Universidad Nacional de Córdoba, Córdoba, 5000, Argentina
Hernández-Fonseca K.:
Departamento de Neuroquímica, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente, Calzada México Xochimilco 101, Col. San Lorenzo Huipulco, D.F., México, 14370, Mexico
Haymal O.B.:
Instituto de Investigación Médica M. y M. Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, 5016, Argentina
Molina J.C.:
Instituto de Investigación Médica M. y M. Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, 5016, Argentina
Facultad de Psicología, Universidad Nacional de Córdoba, Córdoba, 5000, Argentina
Méndez Ubach M.:
Departamento de Neuroquímica, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente, Calzada México Xochimilco 101, Col. San Lorenzo Huipulco, D.F., México, 14370, Mexico
Abate P.:
Facultad de Psicología, Universidad Nacional de Córdoba, Córdoba, 5000, Argentina
Instituto de Investigaciones Psicológicas (IIPsi-CONICET-UNC), Facultad de Psicología, Universidad Nacional de Córdoba, Córdoba, 5000, Argentina
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