Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains
Por:
Navarro-Barranco, Hortensia, Brunner-Mendoza, Carolina, del Rocio Reyes-Montes, Maria, Duarte-Escalante, Esperanza, Toriello, Conchita
Publicada:
1 ene 2019
Resumen:
Metarhizium anisopliae is an anamorphic fungus widely studied due to its
use as a biological control agent. In Mexico, it is mainly used as an
integrated management strategy to control populations of spittlebugs
(Hemiptera: Cercopidae) that affect sugarcane fields and cattle
pastures. In this study, 13 strains isolated from Aeneolamia spp.
(Hemiptera: Cercopidae) from the main areas of sugarcane production of
Mexico and 11 reference strains of Metarhizium from different insect
hosts and geographic origins were evaluated based on their conidial
dimensions, thermotolerance at 15, 25, 30, and 35 degrees C, random
amplification of polymorphic DNA (RAPD) patterns using a combined
analysis with 14 different oligonucleotides and diversity estimators,
and a phylogenetic analysis with the ribosomal RNA internal transcribed
spacer (ITS) region. All strains used in this study showed typical
morphological characteristics corresponding to M. anisopliae and were
able to grow at 25 and 30 degrees C with restricted growth at low (15
degrees C) and high temperatures (35 degrees C). Polymorphism analysis
clustered all strains from Aeneolamia sp. in one well-defined group with
low variability among them. Phylogenetic relationships based on nuclear
ribosomal ITS region sequences recovered a subclade within M. anisopliae
formed by 10 of the Mexican strains.
Filiaciones:
Navarro-Barranco, Hortensia:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, Cd. Universitaria, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Av Univ 3000,Cd Univ, Ciudad De Mexico 04510, Mexico
Brunner-Mendoza, Carolina:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, Cd. Universitaria, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Av Univ 3000,Cd Univ, Ciudad De Mexico 04510, Mexico
del Rocio Reyes-Montes, Maria:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, Cd. Universitaria, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Av Univ 3000,Cd Univ, Ciudad De Mexico 04510, Mexico
Duarte-Escalante, Esperanza:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, Cd. Universitaria, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Av Univ 3000,Cd Univ, Ciudad De Mexico 04510, Mexico
Toriello, Conchita:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, Cd. Universitaria, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Av Univ 3000,Cd Univ, Ciudad De Mexico 04510, Mexico
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