High dietary fat intake induces a microbiota signature that promotes food allergy
Por:
Hussain M., Bonilla-Rosso G., Kwong Chung C.K.C., Bäriswyl L., Rodriguez M.P., Kim B.S., Engel P., Noti M.
Publicada:
1 ene 2019
Resumen:
Background: Diet-induced obesity and food allergies increase in tandem, but a potential cause-and-effect relationship between these diseases of affluence remains to be tested. Objective: We sought to test the role of high dietary fat intake, diet-induced obesity, and associated changes in gut microbial community structure on food allergy pathogenesis. Methods: Mice were fed a high-fat diet (HFD) for 12 weeks before food allergen sensitization on an atopic dermatitis–like skin lesion, followed by intragastric allergen challenge to induce experimental food allergy. Germ-free animals were colonized with a signature HFD or lean microbiota for 8 weeks before induction of food allergy. Food-induced allergic responses were quantified by using a clinical allergy score, serum IgE levels, serum mouse mast cell protease 1 concentrations, and type 2 cytokine responses. Accumulation of intestinal mast cells was examined by using flow cytometry and chloroacetate esterase tissue staining. Changes in the gut microbial community structure were assessed by using high-throughput 16S ribosomal DNA gene sequencing. Results: HFD-induced obesity potentiates food-induced allergic responses associated with dysregulated intestinal effector mast cell responses, increased intestinal permeability, and gut dysbiosis. An HFD-associated microbiome was transmissible to germ-free mice, with the gut microbial community structure of recipients segregating according to the microbiota input source. Independent of an obese state, an HFD-associated gut microbiome was sufficient to confer enhanced susceptibility to food allergy. Conclusion: These findings identify HFD-induced microbial alterations as risk factors for experimental food allergy and uncouple a pathogenic role of an HFD-associated microbiome from obesity. Postdieting microbiome alterations caused by overindulgence of dietary fat might increase susceptibility to food allergy. © 2019 American Academy of Allergy, Asthma & Immunology
Filiaciones:
Hussain M.:
Institute of Pathology, Department of Experimental Pathology, University of Bern, Bern, Switzerland
Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland
Bonilla-Rosso G.:
Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland
Kwong Chung C.K.C.:
Institute of Pathology, Department of Experimental Pathology, University of Bern, Bern, Switzerland
Bäriswyl L.:
Institute of Pathology, Department of Experimental Pathology, University of Bern, Bern, Switzerland
Rodriguez M.P.:
Institute of Pathology, Department of Experimental Pathology, University of Bern, Bern, Switzerland
Kim B.S.:
Division of Dermatology, Department of Medicine, Washington University School of Medicine, St Louis, Mo, United States
Engel P.:
Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland
Noti M.:
Institute of Pathology, Department of Experimental Pathology, University of Bern, Bern, Switzerland
|