ASSESSING THE TAXONOMIC VALIDITY OF AUSTRODIPLOSTOMUM SPP. (DIGENEA: DIPLOSTOMIDAE) THROUGH NUCLEAR AND MITOCHONDRIAL DATA
Por:
Sereno-Uribe A.L., Gómez L.A., Núñez M.O.D., León G.P.-P.D., Garcia-Varela, Martin
Publicada:
1 ene 2019
Resumen:
Adults of the genus Austrodiplostomum are parasites in cormorants of the
New World, whereas metacercariae are parasites from eye globe and brain
of freshwater and brackish water fishes. In this study, specimens of
Austrodiplostomum mordax from South America (type-species) were analyzed
together with other specimens of Austrodiplostomum spp. collected from
several locations across Middle America and North America. Partial DNA
sequences of the mitochondrial gene cytochrome c oxidase subunit I
(COI), the internal transcribed spacers (ITS1, ITS2, and 5.8S gene), and
the D2-D3, domains of the large subunit (LSU) of nuclear ribosomal DNA,
were generated for both developmental stages and compared with available
sequences of Austrodiplostomum spp. Phylogenetic analyses inferred with
each molecular marker using maximum likelihood and Bayesian inference
revealed the existence of 4 lineages representing 2 described species,
A. mordax and Austrodiplostomum compactum (syn. Austrodiplostomum
ostrowskiae) and 2 undescribed species of Austrodiplostomum recognized
in previous studies. The COI haplotype network inferred with 172
sequences detected 28 haplotypes divided into 4 clusters, separating
each other by 33 and 40 substitutions and with a genetic divergence
ranging from 9 to 12%. The largest group included specimens identified
as A. compactum plus those identified as A. ostrowskiae, supporting the
synonymy of both species. As a result, we conclude that A. compactum is
widely distributed across the Americas, in locations of the United
States, Mexico, El Salvador, Honduras, Costa Rica, Venezuela, Peru, and
Brazil. The other 2 undescribed species of the genus Austrodiplostomum
were previously recorded in the United States and now are reported in
Mexico. These 2 species cannot be described because adult forms have not
been found in their definitive hosts. Finally, the species A. mordax has
been found only in some lakes from Argentina, and it was validated in
this study through molecular analyses.
Filiaciones:
Sereno-Uribe A.L.:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, Distrito Federal, CP 04510, Mexico
Gómez L.A.:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, Distrito Federal, CP 04510, Mexico
Universidad Nacional Autónoma de México (UNAM), Posgrado en Ciencias Biológicas, Avenida Universidad 3000, Ciudad Universitaria, Distrito Federal, CP 04510, Mexico
Núñez M.O.D.:
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, Buenos Aires, 1428, Argentina
León G.P.-P.D.:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, Distrito Federal, CP 04510, Mexico
Garcia-Varela, Martin:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, Distrito Federal, CP 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Posgrad Ciencias Biol, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Ciudad Univ,Pabellon 2, RA-1428 Buenos Aires, DF, Argentina
|