Whole-Genome Sequences of Five Acinetobacter baumannii Strains From a Child With Leukemia M2
Por:
Mancilla-Rojano J., Castro-Jaimes S., Ochoa S.A., Del Valle M.B., Luna-Pineda V.M., Bustos P., Laris-González A., Arellano-Galindo J., Parra-Ortega I., Hernández-Castro R., Cevallos M.A., Xicohtencatl-Cortes J., Cruz-Córdova A.
Publicada:
6 feb 2019
Resumen:
Acinetobacter baumannii is an opportunistic pathogen and is one of the
primary etiological agents of healthcare-associated infections (HAIs).
A. baumannii infections are difficult to treat due to the intrinsic and
acquired antibiotic resistance of strains of this bacterium, which
frequently limits therapeutic options. In this study, five A. baumannii
strains (810CP, 433H, 434H, 483H, and A-2), all of which were isolated
from a child with leukemia M2, were characterized through antibiotic
susceptibility profiling, the detection of genes encoding carbapenem
hydrolyzing oxacillinases, pulsed-field gel electrophoresis (PFGE),
multilocus sequence typing (MLST), adherence and invasion assays toward
the A549 cell line, and the whole-genome sequence (WGS). The five
strains showed Multidrug resistant (MDR) profiles and amplification of
the bla(OXA-23) gene, belonging to ST758 and grouped into two PFGE
clusters. WGS of 810CP revealed the presence of a circular chromosome
and two small plasmids, pAba810CPa and pAba810CPb. Both plasmids carried
genes encoding the Sp1TA system, although resistance genes were not
identified. A gene-by-gene comparison analysis was performed among the
A. baumannii strains isolated in this study and others A. baumannii
ST758 strains (HIMFG and INCan), showing that 86% of genes were present
in all analyzed strains. Interestingly, the 433H, 434H, and 483H strains
varied by 8-10 single-nucleotide variants (SNVs), while the A2 and 810CP
strains varied by 46 SNVs. Subsequently, an analysis using BacWGSTdb
showed that all of our strains had the same resistance genes and were
ST758. However, some variations were observed in relation to virulence
genes, mainly in the 810CP strain. The genes involved in the synthesis
of hepta-acylated lipooligosaccharides, the pgaABCD locus encoding
poly-beta-1-6-N-acetylglucosamine, the ompA gene, Csu pili, bap, the
two-component system bfms/bfmR, a member of the phospholipase D family,
and two iron-uptake systems were identified in our A. baumannii strains
genome. The five A. baumannii strains isolated from the child were
genetically different and showed important characteristics that promote
survival in a hospital environment. The elucidation of their genomic
sequences provides important information for understanding their
epidemiology, antibiotic resistance, and putative virulence factors.
Filiaciones:
Mancilla-Rojano J.:
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Mexico City, DF, Mexico
Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico City, Mexico
Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
Castro-Jaimes S.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca, Morelos, Mexico
Centro de Ciencias Genómicas, Programa de Genómica Evolutiva, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Ochoa S.A.:
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Mexico City, DF, Mexico
Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico City, Mexico
Del Valle M.B.:
Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Enfermedades Infeccioses, Mexico City, DF, Mexico
Departamento de Enfermedades Infecciosas, Instituto Nacional de Ciencias Médicas y de Nutrición Salvador Zubirán, Mexico City, Mexico
Luna-Pineda V.M.:
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Mexico City, DF, Mexico
Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico City, Mexico
Bustos P.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca, Morelos, Mexico
Centro de Ciencias Genómicas, Programa de Genómica Evolutiva, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Laris-González A.:
Hosp Infantil Mexico Dr Federico Gomez, Dept Epidemiol, Mexico City, DF, Mexico
Departamento de Epidemiología, Hospital Infantil de México Federico Gómez, Mexico City, Mexico
Arellano-Galindo J.:
Hosp Infantil Mexico Dr Federico Gomez, Lab Infectol, Mexico City, DF, Mexico
Laboratorio de Infectología, Hospital Infantil de México Federico Gómez, Mexico City, Mexico
Parra-Ortega I.:
Hosp Infantil Mexico Dr Federico Gomez, Lab Cent, Mexico City, DF, Mexico
Laboratorio Central, Hospital Infantil de México Federico Gómez, Mexico City, Mexico
Hernández-Castro R.:
Hosp Gen Dr Manuel Gea Gonzalez, Dept Ecol Agentes Patogenos, Mexico City, DF, Mexico
Departamento de Ecología de Agentes Patógenos, Hospital General Dr. Manuel Gea González, Mexico City, Mexico
Cevallos M.A.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca, Morelos, Mexico
Centro de Ciencias Genómicas, Programa de Genómica Evolutiva, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Xicohtencatl-Cortes J.:
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Mexico City, DF, Mexico
Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico City, Mexico
Cruz-Córdova A.:
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Mexico City, DF, Mexico
Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico City, Mexico
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