Poly(vinyl alcohol co-vinyl acetate) as a novel scaffold for mammalian cell culture and controlled drug release
Por:
Villanueva-Flores, Francisca, Miranda-Hernandez, Margarita, Flores-Flores, Jose O., Porras-Sanjuanico, Alberto, Hu, Hailin, Perez-Martinez, Leonor, Ramirez, Octavio T., Palomares, Laura A.
Publicada:
1 may 2019
Resumen:
Tissue engineering requires novel smart materials to sustain cell
growth, tissue regeneration and in situ drug release in a controlled
mode. Also, biocompatible synthesis methods are needed to immobilize
biologically active compounds. Poly(vinyl alcohol co-vinyl acetate)
(PAcVA) was synthesized at 37 degrees C using glutaraldehyde (GA) as a
crosslinking agent. The mechanical characteristics of the polymer were
manipulated by varying crosslinking degrees using different GA
concentrations. Materials with Young's modules similar to soft tissues,
adequate for tissue engineering, were obtained. PAcVA was a
pH-responsive material with maximum swelling at pH 5.8. When hydrated,
PAcVA was electro-responsive. Fluorescein was used as a model molecule
to characterize the releasing properties of the polymer. Effective
diffusivities were a function of the crosslinking degree. Release rates
were proportional to temperature and were faster at lower GA contents.
According to a fit to the Korsmeyer-Peppas' model, diffusion at 5 and
10% GA was Fickian, but at 20% GA, diffusion was abnormal. To promote
cell attachment and neutralize free aldehyde groups, PAcVA hydrogels
were covered with poly-l-lysine and laminin, which supported growth of
lung carcinoma and mouse hypothalamic cells without signs of
cytotoxicity or oxidative stress. An intelligent low-cost hydrogel with
properties that can be easily modulated was synthesized and fully
characterized. Its properties make it suitable for tissue engineering
applications, as they mimic the mechanical properties of natural
tissues.
Filiaciones:
Villanueva-Flores, Francisca:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Ave. Universidad 2001. Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Ave Univ 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico
Miranda-Hernandez, Margarita:
Instituto de Energías Renovables, Universidad Nacional Autónoma de México, Priv. Xochicalco S/N, Temixco, Morelos 62580, Mexico
Univ Nacl Autonoma Mexico, Inst Energias Renovables, Priv Xochicalco S-N, Temixco 62580, Morelos, Mexico
Flores-Flores, Jose O.:
Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior S/N. Ciudad Universitaria, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Ciencias Aplicadas & Tecnol, Circuito Exterior S-N,Ciudad Univ, Mexico City 04510, DF, Mexico
Porras-Sanjuanico, Alberto:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Ave. Universidad 2001. Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Ave Univ 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico
Hu, Hailin:
Instituto de Energías Renovables, Universidad Nacional Autónoma de México, Priv. Xochicalco S/N, Temixco, Morelos 62580, Mexico
Univ Nacl Autonoma Mexico, Inst Energias Renovables, Priv Xochicalco S-N, Temixco 62580, Morelos, Mexico
Perez-Martinez, Leonor:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Ave. Universidad 2001. Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Ave Univ 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico
Ramirez, Octavio T.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Ave. Universidad 2001. Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Ave Univ 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico
Palomares, Laura A.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Ave. Universidad 2001. Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Ave Univ 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico
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