Simultaneous Detection of Beta and Gamma Human Herpesviruses by Multiplex qPCR Reveals Simple Infection and Coinfection Episodes Increasing Risk for Graft Rejection in Solid Organ Transplantation
Por:
Sanchez-Ponce, Yessica, Varela-Fascinetto, Gustavo, Carlos Romo-Vazquez, Jose, Lopez-Martinez, Briceida, Luis Sanchez-Huerta, Jose, Parra-Ortega, Israel, Fuentes-Panana, Ezequiel M., Morales-Sanchez, Abigail
Publicada:
1 dic 2018
Resumen:
Herpesviruses are common components of the human microbiome that become
clinically relevant when a competent immunosurveillance is compromised,
such as in transplantation. Members of the beta and gamma subfamilies
are associated with a wide diversity of pathologies, including end-organ
disease and cancer. In this study, we developed a multiplex qPCR
technique with high specificity, sensitivity, efficiency and
predictability that allowed the simultaneous detection and
quantification of beta and gamma human herpesviruses. The technique was
tested in a cohort of 34 kidney- or liver-transplanted pediatric
patients followed up for up to 12 months post-transplant. Viral load was
determined in 495 leukocyte-plasma paired samples collected bi-weekly or
monthly. Human herpesvirus (HHV) 7 was the herpesvirus most frequently
found in positive samples (39%), followed by Epstein-Barr virus (EBV)
(20%). Also, EBV and HHV7 were present in the majority of coinfection
episodes (62%). The share of positive samples exclusively detected
either in leukocytes or plasma was 85%, suggesting that these
herpesviruses tended to take a latent or lytic path in an exclusive
manner. Infection by human cytomegalovirus (HCMV) and HHV6, as well as
coinfection by EBV/HHV7 and EBV/HHV6/HHV7, were associated with graft
rejection (RR = 40.33 (p = 0.0013), 5.60 (p = 0.03), 5.60 (p = 0.03) and
17.64 (p = 0.0003), respectively). The routine monitoring of beta and
gamma herpesviruses should be mandatory in transplant centers to
implement preventive strategies.
Filiaciones:
Sanchez-Ponce, Yessica:
Childrens Hosp Mexico Federico Gomez, Res Unit Virol & Canc, Mexico City 06720, DF, Mexico
Univ Nacl Autonoma Mexico, Postgrad Program Biol Sci, Mexico City 04510, DF, Mexico
Varela-Fascinetto, Gustavo:
Childrens Hosp Mexico Federico Gomez, Dept Transplantat, Mexico City 06720, DF, Mexico
Carlos Romo-Vazquez, Jose:
Childrens Hosp Mexico Federico Gomez, Dept Nephrol, Mexico City 06720, DF, Mexico
Lopez-Martinez, Briceida:
Childrens Hosp Mexico Federico Gomez, Subdirect Diagnost Auxiliary Serv, Mexico City 06720, DF, Mexico
Luis Sanchez-Huerta, Jose:
Childrens Hosp Mexico Federico Gomez, Dept Clin Lab, Mexico City 06720, DF, Mexico
Parra-Ortega, Israel:
Childrens Hosp Mexico Federico Gomez, Dept Clin Lab, Mexico City 06720, DF, Mexico
Fuentes-Panana, Ezequiel M.:
Childrens Hosp Mexico Federico Gomez, Res Unit Virol & Canc, Mexico City 06720, DF, Mexico
Morales-Sanchez, Abigail:
Childrens Hosp Mexico Federico Gomez, Res Unit Virol & Canc, Mexico City 06720, DF, Mexico
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