A morphological and molecular study of adults and metacercariae of Hysteromorpha triloba (Rudolpi, 1819), Lutz 1931 (Diplostomidae) from the Neotropical region
Por:
Sereno-Uribe, A. L., Lopez-Jimenez, A., Andrade-Gomez, L., Garcia-Varela, M.
Publicada:
1 ene 2019
Resumen:
Adults of Hysteromorpha triloba (Rudolpi, 1819), Lutz, 1931 inhabit
primarily the intestine of cormorants across the globe, whereas
metacercariae have been found in the body cavity of freshwater fishes of
the families Cyprinidae, Ictaluridae, Ariidae, Pimelodidae and
Catostomidae. In this study, adults and metacercariae identified as H.
triloba were collected from the Neotropical cormorant (Nannopterum
brasilianus) and from the Mexican tetra fish (Astyanax mexicanus) from
the Gulf of Mexico and Pacific Ocean slopes in the Neotropical region.
Partial DNA sequences of the mitochondrial gene cytochrome c oxidase
subunit I (cox 1) and the internal transcribed spacers (ITS1, 5.8S and
ITS2) of nuclear ribosomal DNA were generated for both developmental
stages, and were compared with available sequences of H. triloba from
the Nearctic region. The genetic divergence between metacercariae and
adults of H. triloba from the Neotropical and Nearctic region (Canada)
associated with the double-crested cormorant (Nannopterum auritus),
ranged from 0 to 5.5% for cox 1 and from 0 to 0.2% for ITS.
Phylogenetic analyses inferred with both molecular markers using maximum
likelihood and Bayesian inference placed the adults and metacercariae in
a single clade, confirming that both stages are conspecific. Our data
confirmed that H. triloba is a widely distributed species across the
Americas, parasitizing both the Neotropical and Nearctic cormorants in
Argentina, Brazil, Venezuela, Mexico, USA and Canada.
Filiaciones:
Sereno-Uribe, A. L.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, Ciudad de México, C.P. 04510, Mexico
Lopez-Jimenez, A.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, Ciudad de México, C.P. 04510, Mexico
Andrade-Gomez, L.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, Ciudad de México, C.P. 04510, Mexico
Garcia-Varela, M.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, Ciudad de México, C.P. 04510, Mexico
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